Publication: H-RAS gene expression in human multinodular goiter
Authors
Golbert, L. ; Kolling, J.H.G. ; Leitão, A.H. ; Martins, L. ; Kimura, E.T. ; Maia, A.L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The RAS protooncogene has an important,
although not yet established role in thyroid neoplasia. In
this study, we evaluated the H-RAS mRNA and protein
levels in human samples of nontoxic and toxic
multinodular goiter samples, according to serum TSH
levels. The mean of H-RAS mRNA levels in nodules of
nontoxic nodular goiter were significantly increased
compared to nonnodular tissue (1.49±1.21 vs. 0.94±0.81
AU, P=0.016). Nine of the 18 specimens (50%) of
nontoxic multinodular goiter exhibited increased levels
of H-RAS mRNA. The increased H-RAS mRNA levels
were paralleled by inRAcreased H-Ras protein levels in
about 90% of the cases. Interestingly, no differences
were observed in H-RAS expression between nodules
and adjacent nonnodular tissue in toxic nodular goiters
(0.58±0.27 vs. 0.58±0.20 AU, P=0.88). None of the 10
samples from toxic multinodular goiters exhibited
overexpression of H-RAS. The H-RAS expression was
positively correlated with thyroglobulin expression
(r2=0.51; P=0.04). In conclusion, we demonstrated
increased levels of H-RAS mRNA and protein in samples
of nontoxic multinodular goiter, indicating that it might
be involved in goiter pathogenesis. In contrast, H-RAS
overexpression was not detected in any of the samples of
toxic multinodular goiter, suggesting different
mechanisms for cell proliferation in nodular goiter
according to thyroid status.
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