Salivary testosterone measurements in growing pigs: validation of an automated chemiluminescent immunoassay and its possible use as an acute stress marker

Escribano Tortosa, Damián; Fuentes Rubio, María; Cerón Madrigal, José Joaquín

Publication:
Salivary testosterone measurements in growing pigs: validation of an automated chemiluminescent immunoassay and its possible use as an acute stress marker

dc.contributor.author	Escribano Tortosa, Damián
dc.contributor.author	Fuentes Rubio, María
dc.contributor.author	Cerón Madrigal, José Joaquín
dc.contributor.department	Producción Animal
dc.date.accessioned	2025-01-16T07:48:42Z
dc.date.available	2025-01-16T07:48:42Z
dc.date.issued	2014-08
dc.description	© 2014 Elsevier Ltd. This document is the Published version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/10.1016/j.rvsc.2014.04.001	es
dc.description.abstract	This study aimed to validate the use of an automated chemiluminescent immunoassay analyser for sal- ivary testosterone measurements in growing pigs and study how circadian pattern during daytime and stress can influence its values. The test method had intra- and inter-assay coefficient of variation lower than 10%. The method showed good linearity and recovery, and detection limits were low enough to detect salivary testosterone levels. No significant differences were observed in testosterone concentrations at different sampling time, and age and gender did not influence circadian pattern. In addition, this assay was used to quantify testosterone in two models of acute stress and, in both cases, significant increases (P < 0.01) in salivary testosterone were detected. Therefore, the automated assay system tested for porcine testosterone determinations would be suitable for its use in saliva samples and, furthermore, salivary testosterone levels could be used as a possible marker of acute stress in pigs.	es
dc.format	application/pdf	es
dc.format.extent	6	es
dc.identifier.citation	Research in Veterinary Science, 2014, Vol. 97, Issue 1, pp. 20-25
dc.identifier.doi	https://doi.org/10.1016/j.rvsc.2014.04.001
dc.identifier.issn	Print: 0034-5288
dc.identifier.issn	Electronic: 1532-2661
dc.identifier.uri	http://hdl.handle.net/10201/148542
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	This study was supported by a grant from the University of Murcia and by the Spanish Ministry of Education and Science (AGL 2009-08509). Proyecto: Nuevos biomarcadores de estrés en saliva de ganado porcino.	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S003452881400099X?via%3Dihub
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	Automated chemiluminescent immunoassay
dc.subject	Pig
dc.subject	Salivary testosterone
dc.subject	Acute stress
dc.subject.other	CDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos	es
dc.title	Salivary testosterone measurements in growing pigs: validation of an automated chemiluminescent immunoassay and its possible use as an acute stress marker	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es