Publication: Salivary testosterone measurements in growing pigs: validation of an automated chemiluminescent immunoassay and its possible use as an acute stress marker
Authors
Escribano Tortosa, Damián ; Fuentes Rubio, María ; Cerón Madrigal, José Joaquín
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.rvsc.2014.04.001
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info:eu-repo/semantics/article
Description
© 2014 Elsevier Ltd. This document is the Published version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/10.1016/j.rvsc.2014.04.001
Abstract
This study aimed to validate the use of an automated chemiluminescent immunoassay analyser for sal- ivary testosterone measurements in growing pigs and study how circadian pattern during daytime and stress can influence its values. The test method had intra- and inter-assay coefficient of variation lower than 10%. The method showed good linearity and recovery, and detection limits were low enough to detect salivary testosterone levels. No significant differences were observed in testosterone concentrations at different sampling time, and age and gender did not influence circadian pattern. In addition, this assay was used to quantify testosterone in two models of acute stress and, in both cases, significant increases (P < 0.01) in salivary testosterone were detected. Therefore, the automated assay system tested for porcine testosterone determinations would be suitable for its use in saliva samples and, furthermore, salivary testosterone levels could be used as a possible marker of acute stress in pigs.
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Citation
Research in Veterinary Science, 2014, Vol. 97, Issue 1, pp. 20-25
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