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Ultrastrucmturaiml munogold labelling of vimentin filaments on postembedding ultrathin sections of arachnoid villi and meningiomas

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dc.contributor.authorYamashima, Tetsumori
dc.contributor.authorTachibana, Osamu
dc.contributor.authorNitta, Hisashi
dc.contributor.authorYamaguchi, Narihito
dc.contributor.authorYamashita, Junkoh
dc.date.accessioned2011-01-18T12:37:20Z
dc.date.available2011-01-18T12:37:20Z
dc.date.issued1989
dc.description.abstractAn immunoelectron microscopic technique for the labelling of vimentin intermediate filaments on postembedding ultrathin sections is reported. Arachnoid villi obtained at autopsy and meningiomas at surgery were fixed in 1% paraformaldehyde for 30 minutes, embedded without postfixation in Epon-Araldite mixture and polymerized at 37OC for 3 weeks. Ultrathin sections were etched in 2% KOH for 3 minutes and incubated with anti-vimentin monoclonal antibodies which were subsequently labelled with goat anti-mouse IgG coupled to colloidal golds. Al1 of these labelling procedures were consistently performed within 4 hours. In both arachnoidal and meningioma cells, immunogolds preferentially decorated the intermediate filaments in proportion to the concentration. Very few gold particles were seen over the nucleus, Golgi zone, mitochondria and the extracellular connective tissue fibres. The present technique may be applied to the immunogold labelling of intermediate filaments on postembedding ultrathin sections.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/17973
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectArachnoid villuses
dc.subjectElectron microscopyes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleUltrastrucmturaiml munogold labelling of vimentin filaments on postembedding ultrathin sections of arachnoid villi and meningiomases
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol. 4, nº 1 (1989)