Publication: Ultrastrucmturaiml munogold labelling of vimentin
filaments on postembedding ultrathin sections
of arachnoid villi and meningiomas
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Date
1989
Authors
Yamashima, Tetsumori ; Tachibana, Osamu ; Nitta, Hisashi ; Yamaguchi, Narihito ; Yamashita, Junkoh
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
An immunoelectron microscopic technique
for the labelling of vimentin intermediate filaments on
postembedding ultrathin sections is reported. Arachnoid
villi obtained at autopsy and meningiomas at surgery
were fixed in 1% paraformaldehyde for 30 minutes,
embedded without postfixation in Epon-Araldite
mixture and polymerized at 37OC for 3 weeks. Ultrathin
sections were etched in 2% KOH for 3 minutes and
incubated with anti-vimentin monoclonal antibodies
which were subsequently labelled with goat anti-mouse
IgG coupled to colloidal golds. Al1 of these labelling
procedures were consistently performed within 4 hours.
In both arachnoidal and meningioma cells, immunogolds
preferentially decorated the intermediate filaments in
proportion to the concentration. Very few gold particles
were seen over the nucleus, Golgi zone, mitochondria
and the extracellular connective tissue fibres. The
present technique may be applied to the immunogold
labelling of intermediate filaments on postembedding
ultrathin sections.
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