Publication: Techniques and application of microscopic radioautography
Authors
Nagata, T.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The techniques for light and electron
microscopic radioautography developed in our
laboratory are described. The outlines of the applications
to various tissues and organs are also briefly
summarized.
First, the techniques are explained in detail. These
techniques can be divided into two categories, Le.,
chemical fixation followed by wet-mounting radioautography
and cryo-fixation followed by dry-mounting
radioautography. The former is limited in application to
only insoluble compounds, while the latter is universally
applicable to any kind of compounds including soluble
compounds. Both procedures, the chemical fixation wetmounting
procedure and the cryo-fixation dry-mounting
procedure at light and electron microscopic levels are
described.
Next, the applications of both the procedures to
various compounds are briefly mentioned. Chemical
fixation with glutaraldehyde and osmium tetroxide
followed by wet-mounting radioautography demonstrates
insoluble macromolecular synthesis such as
nucleic acids (both DNA and RNA), proteins, glucides
and lipids. On the other hand, cyro-fixation followed by
dry-mounting radioautography demonstrates soluble
small molecular compounds such as macromolecular
precursors, hormones, neurotransmitters, vitamins,
inorganic compounds and drugs or toxins.
These procedures are expected to be applied for
various inorganic and organic compounds in living
organisms to elucidate the sites of their incorporation,
synthesis and discharge.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.