Publication: Toll-like receptor 4 in normal and inflamed
lungs and other organs of pig, dog and cattle
Authors
Wassef, A. ; Janardhan, K. ; Pearce, J.W. ; Singh, B.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Bacterial diseases, especially those of the
lung caused by Gram-negative bacteria, inflict
significant economic loss associated with mortality and
morbidity in domestic animals. Toll-like receptor 4
(TLR4) has recently been recognized as a major receptor
for cellular interactions with lipopolysaccharides derived
from Gram-negative bacteria. However, there are no data
on the expression of TLR4 in various organs of domestic
animals. We performed immunohistochemistry and
immuno-gold electron microscopy to localize TLR4 in
lung and seven other organs from normal pig, dog and
calf (n=2 each) and in inflamed lungs from calves (n=4)
challenged with Mannheimia hemolytica. The data show
TLR4 in macrophages in lung, small intestine, liver and
spleen in all the species and pulmonary intravascular
macrophages in calves and pigs. Epithelium in lung,
small intestine, cornea and convoluted and straight renal
tubules was stained for TLR4. Vascular endothelium of
large blood vessels only in lungs and skin was positive,
and skeletal muscles were negative for TLR4. In
inflamed lungs, airway epithelium showed reduced
staining for TLR4 while staining in macrophages
remained unaltered. These are the first
immunocytochemical data on TLR4 expression in
domestic animal species and show similarity in TLR4
staining in macrophages, epithelium and vascular
endothelium among dog, pig and cattle.
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