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Reduction of PGRN increased fibrosis during skin wound healing in mice

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dc.contributor.authorLi, Shan-shan
dc.contributor.authorZhang, Mei Xiang
dc.contributor.authorWang, Yue
dc.contributor.authorWang, Wei
dc.contributor.authorZhao, Chun Ming
dc.contributor.authorSun, Xiao Ming
dc.contributor.authorDong, Guo Kai
dc.contributor.authorLi, Zhou Ru
dc.contributor.authorYin, Wen Jiang
dc.contributor.authorZhu, Bo
dc.contributor.authorCai, Hong Xing
dc.date.accessioned2022-07-05T07:29:30Z
dc.date.available2022-07-05T07:29:30Z
dc.date.issued2019
dc.description.abstractProgranulin (PGRN) is a multi-functional growth factor known to be involved in regulating of development, cell cycle progression, cell motility, tumorigenesis and angiogenesis. Research has revealed that PGRN is a crucial mediator of skin wound healing. Nonetheless, the role of PGRN in the fibrosis process of cutaneous wound healing has not been identified. In the present study, mice with excisional wounds were treated with si-m-PGRN or physiological saline. We observed the expression of PGRN in intact and post-injury skin by immunohistochemistry. Tissue sections of skin around the wound were performed by hematoxylin and eosin and masson's trichrome staining. After PGRN knockdown by siRNA, the expression of PGRN, collagen I (Col I), small mothers against decapentaplegic homolog 3 (Smad3), phosphorylated Smad3 (P-Smad3), transforming growth factor (TGF)-β1 and TGF-β receptor I (TβRI) were detected by real-time reverse transcription polymerase chain reaction (RT-qPCR) or Western blot. PGRN mRNA and protein expressions were increased after insult and remained above that of intact skin through day 20. Down-regulation of PGRN augmented fibrosis area, skin thickness and the expression of Col I. In addition, reduction of PGRN considerably increased the expression of TGF-β1, TβRI, Smad3 and P-Smad3. These results indicate that PGRN knockdown enhances the fibrosis degree, probably via the TGF-β/Smad signaling pathway.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.citationHistology and Histopathology, Vol.34, nº7, (2019)
dc.identifier.doiDOI: 10.14670/HH-18-076
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/122105
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCutaneous wound healinges
dc.subjectFibrosises
dc.subjectPGRNes
dc.subjectTGF-β/Smades
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleReduction of PGRN increased fibrosis during skin wound healing in micees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.34, nº7 (2019)