Publication: New serum-derived albumin scaffold seeded
with adipose-derived stem cells and olfactory
ensheathing cells used to treat spinal cord injured rats
Authors
Ferrero Gutiérrez, Amaia ; Menéndez Menéndez, Yolanda ; Álvarez Viejo, Maria ; Meana, Álvaro ; Otero, Jesús
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Recent advances in spinal cord injury (SCI)
research and cell culture techniques and biomaterials
predict promising new treatments for patients with SCI
or other nerve injuries. Biomaterial scaffolds form a
substrate within which cells are instructed to form a
tissue in a controlled manner. This study was designed to
assess axon regeneration and locomotor recovery in rats
with spinal cord injury treated with a novel serumderived
albumin scaffold seeded with adipose derived
stem cells (ADSCs) and olfactory ensheathing cells
(OECs). OECs are considered promising candidates for
the treatment of SCI, and ADSCs have the ability to
differentiate into neural lineages. In vitro experiments
revealed that ADSCs and OECs adhered to the scaffold,
remained viable and expressed specific markers of their
cell types when cultured in the scaffold. Rats treated
with scaffold plus cells showed locomotor skills at
several time points from 45 days post-injury that were
improved over those recorded in control injured,
untreated animals. Astrocytic scars and tissue
regeneration, identified using histological and
immunohistochemical techniques, revealed that although
the scaffold itself appeared to play a significant role in
reducing glial scar formation and filling of the lesion
cavity with cells, the presence of ADSCs and OECs in
the scaffold led to the appearance of cells expressing
markers of neurons and axons at the injury site. Our
findings point to the clinical feasibility of an albumin
scaffold seeded with ADSCs and OECs as a treatment
candidate for use in spinal cord injury repair studies.
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Citation
Histology and histopathology, Vol. 28, n.º 1 (2013)
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