Publication: Expression of muscarinic acetylcholine
receptors (M1-, M2-, M3- and M4-type) in the
neuromuscular junction of the newborn and adult rat
Authors
García, N . ; Santafe, M. ; Salmon, I. ; Lanuza, M.A. ; Tomas, J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Using intracellular recording and
immunohistochemistry, we studied the presynaptic
muscarinic autoreceptor subtypes controlling ACh
release in the neuromuscular junctions of the newborn
(3-6 days postnatal) and adult (30-40 days) rat. In the
Levator auris longus muscles of both newborn and adult
rats, acetylcholine release was modified by the M1-
receptor selective antagonists pirenzepine (10 µM) and
MT-7 (100 nM) and by the M2-receptor selective
antagonists methoctramine (1 µM) and AF-DX 116 (10
µM). The M4-receptor selective antagonists tropicamide
(1 µM) and MT-3 (100 nM) can also modify the
neurotransmitter release in certain synapses of the
newborn muscles. The neurotransmitter release was not
altered by the M3-receptor selective antagonist 4-DAMP
(1 µM) in the adult or newborn rats. However, we
directly demonstrate by immunocytochemistry the
presence of these receptors in the motor endplates and
conclude that M1-, M2-, M3- and M4-type muscarinic
receptors are present in all the neuromuscular junctions
of the rat muscle both in newborn and adult animals.
These receptors may be located in the perisynaptic glial
cell as well as at the nerve terminals.
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