Publication: Assessment of blood-retina1 barrier integrity
Authors
Vinores, S.A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The blood-retina1 barrier consists of two
components which are comprised of the retinal vascular
endothelium and the retinal pigment epithelium,
respectively. Its functional integrity can be recognized
by tight junctions between these cells with a paucity of
endocytic vesicles within them and the presence of the
molecules that regulate the ionic and metabolic gradients
that constitute the barrier. The banier is compromised in
severa1 disease processes and by a variety of agents, but
in most cases the location and mechanism for barrier
failure is not understood. Perfusion with a variety of
radiolabeled tracer molecules, vitreous fluorophotometry,
or magnetic resonance imaging can be used to
quantitate blood-retina1 barrier leakage. Fluorescein
angiography or magnetic resonance imaging can localize
sites of leakage in vivo with limited resolution. Evans
blue dye can be used to visualize blood-retina1 barrier
failure in gross pathological specimens and immunohistochemical
labeling of serum proteins such as
albumin or fibrinogen can be used to localize sites of
blood-retina1 barrier breakdown by light microscopy.
Tracers such as horseradish peroxidase, microperoxidase,
or lanthanum, or the immunocytochemical
demonstration of albumin can be used to reveal bloodretinal
barrier breakdown at the ultrastructural leve1 and
provide insights into the mechanisms involved. This
review discusses the advantages and lirnitations of each
of these methods to aid in selection of the appropriate
techniques to derive the desired information.
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