Publication: Fibroblast activation protein-alpha knockdown suppresses prostate cancer cell invasion and proliferation
Authors
An, Jiali ; Hou, Dingkun ; Wang, Lei ; Wang, Lili ; Yang, Yuanyuan ; Wang, Haitao
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
publication.page.editor
publication.page.department
DOI
https://doi.org/10.14670/HH-18-430
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Background. Prostate cancer is one of the
most common malignant tumors of the male
genitourinary system. Fibroblast activation protein alpha
(FAP-α) overexpression has been shown to occur in a
wide range of tumors. However, the specific mechanism
of FAP-α in the development of prostate cancer has not
been reported.
Methods. In this study, real-time quantitative PCR
(qRT-PCR) was used to detect the relative expression of
FAP-α mRNA in prostate cancer cell lines (PC-3,
LNCaP, and DU145) and human normal prostate
epithelial cell line RWPE-1. Small interfering RNA
(siRNA) targeting FAP-α and vectors expressing
exogenous FAP-α were transfected to prostate cancer
cells (LNCaP and DU145) to investigate the function of
FAP-α. BALB/c nude mice were injected with DU145
cells which were transfected with NC-siRNA, FAP-αsiRNA-1, or FAP-α-siRNA-2.
Results. Compared to adjacent normal tissues, FAPα protein and mRNA levels in prostate cancer tissues
increased significantly (P<0.05). Compared to patients
with high FAP-α mRNA levels, patients with low FAP-α
mRNA levels had a significantly higher survival rate
(χ2=5.050, log-rank P=0.025). Overexpression of FAP-α
in LNCaP cells markedly inhibited cell apoptosis, and
promoted cell invasion and proliferation. In contrast,
knockdown of FAP-α expression in DU145 cells can
significantly reduce invasion, proliferation, and promote
apoptosis in prostate cancer. Immunofluorescence assay
further indicated that down-regulation of FAP-α could
suppress the nuclear translocation of β-catenin. An in
vivo study found that compared with the NC-siRNA
group, the tumor weight and tumor volume in the FAPα-siRNA-1 and FAP-α-siRNA-2 groups were
significantly decreased.
Conclusions. In conclusion, down-regulation of
FAP-α can inhibit the invasion and proliferation of
prostate cancer. Our study provides a theoretical basis
for the targeted treatment of prostate cancer.
publication.page.subject
Citation
Histology and Histopathology Vol. 37, nº6 (2022)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/