Publication: Comparative cytokeratin distribution patterns in cholesteatoma epithelium
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Date
2007
Authors
Olszewska, E. ; Sudhoff, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Cytokeratins (CKs) are known as the
intermediate filament proteins of epithelial origin. Their
distribution in human epithelia is different according to
the type of epithelium, state of growth and
differentiation. We used monoclonal mouse antibodies
against cytokeratins to study CK expression in the
following human tissues: cholesteatoma, middle ear
mucosa, glandular epithelium, and meatal ear canal
epithelium. Immunohistochemical processing was
performed using the labeled steptavidin peroxidase
method to demonstrate the presence of CKs in cells of
human epidermis. Positive reaction was obtained for
CK4, CK34ßE12, CK10, CK14 in skin and
cholesteatoma epithelium. However, a more extensive
positive reaction with those CKs was observed in cholesteatoma epithelium. Positive immunoreactivity
was seen with anti- CK19 in the glandular epithelium.
Middle ear mucosa specimens revealed positive
immunoreactivity with the antibodies against CK4. The
expression of CK4 was definitely positive within the
basal layers of the epidermis. The glandular epithelium
showed no positive reaction with anti- CK4, anti-
CK34ßE12, anti- CK14 and anti-CK10. Immunohistochemistry
for CK18 showed no reaction in all
examined tissues. Cholesteatoma is known as a
proliferative disease in the middle ear which
pathogenesis is not completely understood.
Keratinocytes express hyperproliferation- associated
CKs and after reaching the suprabasal layers they finally
undergo apoptosis creating keratinous debris.
Cytokeratin expression observed in the epithelium
explains proliferative behavior of cholesteatoma which
is associated with increased keratinocyte migration.
Cytokeratins can be used as potential proliferative
markers. It can also allow for searching the usefulness of
inhibiting regulators in the treatment of
hyperproliferative diseases.
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