Publication: Changes of calcium/calmodulin-dependent protein kinase II expression in dorsal root ganglia during maturation in long-term diabetes
Authors
Ferhatovic, Lejla ; Jelicic Kadic, Antonia ; Boric, Matija ; Puljak, Livia
item.page.secondaryauthor
item.page.director
Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Calcium/calmodulin-dependent protein
kinase II (CaMKII) is considered one of the key
intracellular signaling proteins for development of
neuropathy. We analyzed the expression of total CaMKII
(tCaMKII) and its alpha, beta, gamma and delta isoforms
in dorsal root ganglia (DRG) in a rat model of Diabetes
mellitus type I (DM1), 6 months and 1 year after
diabetes induction. Diabetes was induced with
streptozotocin and confirmed by measuring glucose
levels and weight increase. Immunohistochemistry was
performed for detection of tCaMKII and its isoforms in
L4 and L5 DRGs. A significant decrease of CaMKII
alpha and beta isoforms was noted 6 months after
diabetes induction, while CaMKII gamma and delta
were significantly decreased after 12 months in diabetic
rats compared to controls. Analysis of neuronal
subgroups based on the neuronal diameter revealed that
the expression of alpha, beta and delta isoforms
decreased only in small-diameter neurons. In conclusion,
a significant decrease of specific CaMKII isoforms in
small-diameter DRG neurons may suggest involvement
of CaMKII alpha, beta and delta in the development of
complex events responsible for the development of
neuropathy in long-term diabetes during maturation.
CaMKII is a part of the neuronal pathway that regulates
the firing properties of excitable cells, especially
neurons, and decreased CaMKII activity may be
responsible for generation of aberrant signals,
hyperalgesia and neuropathic pain.
Citation
Histology and Histopathology, vol. 29, nº 5, (2014)
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.