Publication: Melatonin promotes self-renewal and nestin expression in neural stem cells from the retina
Authors
Gao, Yuhua ; Ma, Li ; Bai, Chunyu ; Zhang, Xiangyang ; Yang, Wancai
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
DOI: 10.14670/HH-18-065
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Although melatonin has been shown to
exhibit a wide variety of biological functions, its effects
on promoting self-renewal in retinal stem cells remain
unknown. We found that melatonin can significantly
increase proliferation and enhance expression of a stem
cell marker, nestin, in retinal neural stem cells (NSCs)
via melatonin receptor 1 (MT1). The ERK pathway
inhibitor SCH772984 and TGF-β pathway inhibitor
SB431542 were used to study the melatonin-mediated
molecular mechanisms of cell proliferation in NSCs. The
results revealed a novel molecular mechanism of
melatonin promotion of self-renewal of NSCs in which a
chain reaction in the ERK and TGF-β/Smad pathways
promotes self-renewal and transcription of nestin. In
addition, dual-luciferase assays revealed that Smad4
directly regulated nestin transcription after melatonin
treatment in NSCs. These findings revealed novel
mechanisms through which the ERK pathway cooperates
with the Smad pathway to regulate self-renewal in NSCs
to enhance nestin expression.
publication.page.subject
Citation
Histology and Histopathology, Vol.34, nº6, (2019)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/