Publication: Histological and morphometrical evaluation of the urethral wall after bioresorbable stent implantation in male New Zealand White Rabbits: A preliminary study
Authors
Skonieczna-Kurpiel, Joanna ; Madej, Jan P. ; Klekiel, Tomasz ; Mackiewicz, Agnieszka ; Będziński, Romuald ; Noszczyk-Nowak, Agnieszka ; Piasecki, Tomasz
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-18-722
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info:eu-repo/semantics/article
Description
Abstract
The aim of the study was the histological and
morphometrical evaluation of the urethral wall at three
time points after bioresorbable stent implantation in
male New Zealand White Rabbits. The research was
performed on 26 male New Zealand White rabbits aged
3-4 months and weighing 2.1-3.0 kg. Two models of
bioresorbable sodium alginate-based stents were
developed and implanted into the urethral lumen for one
(T1), three (T3), and six weeks (T6). Sections of 5 µm
thickness were cut from the urethra at intervals of 2 mm.
The sliced sections were stained with hematoxylin-eosin
(H&E), Van Gieson's (VG), Von Kossa, and Movat–
Russell modified pentachrome (MOVAT) staining
methods. The study provided valuable information for
future models of urethral stents. The first model of the
stent failed to fit the requirements due to inadequate
mechanical properties. It curled up on itself losing the
ability to adhere to the animals’ urethra and was
bioresorbed three weeks after implantation. The more
rigid no. 2 stent was effective in widening the urethral
lumen but did not biodegrade during the experiment. A
comprehensive assessment of the second model’s
properties of biosorption and biointegration requires an
extended observation of at least 12 months for an in
depth morphological analysis. Stent migration is not
likely to be caused solely by the mechanical properties
of the urethra or urinary flow but mainly by muscle
contraction of the organ wall.
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