Publication: Does coenzyme-Q have a protective effect against
atorvastatin induced myopathy? A histopathological
and immunohistochemical study in albino rats
Authors
Khalil, Mahmoud Salah ; Khamis, Nehal ; Al-drees, Abdulmajeed ; Abdulghani, Hamza Mohammad
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Publisher
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DOI
https://doi.org/10.14670/HH-30.383
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info:eu-repo/semantics/article
Description
Abstract
Introduction. In addition to their lipidlowering effect, statins have pleiotropic effects that may
extend their use to the treatment and prevention of
various other diseases such as cancer, osteoporosis,
multiple sclerosis, rheumatoid arthritis, type 2 diabetes,
and Alzheimer’s disease. Consequently, the number of
patients taking statins is expected to increase. A side
effect of statins, statin-induced myopathy, which may
result from reduced muscular coenzyme Q10 levels,
limits their use. The current study investigates if
supplementing with CoQ10 could ameliorate statin
induced myopathy. Materials and Methods. Forty adult
male albino rats were randomized into 4 groups, with 10
rats per group. The following was administered to the
rats using oral gavage for 4 weeks: Group 1: 2 ml of 0.5%
carboxymethyl cellulose once daily. Group 2: 100
mg/kg/ day coenzyme Q10 dissolved in 2 ml of cotton
seed oil. Group 3: 10 mg/kg once daily atorvastatin
dissolved in 0.5% carboxymethyl cellulose. Group 4:
concomitantly received CoQ10 and atorvastatin similar
to groups 2 and 3 respectively. Plasma creatine kinase
levels were measured by using spectrophotometer. The
right extensor digitorum longus muscle sections were
stained for histological (Haematoxylin & Eosin, Masson
trichrome and Phosphotungstic acid haematoxylin) and
immunohistochemical (cytochrome C and Bax)
examinations. Quantitative measures of cytochrome C
and Bax were carried out using image analyzer.
Results. Atorvastatin induced increased total creatine
kinase, skeletal muscle variations in the sizes and
shapes, necrosis, disorganization, nuclear pyknosis,
karyorrhexis, karyolysis, dismantled plasma membrane,
excess collagen fibers and lipid deposition in addition to
loss of cross striation. Atorvastatin increased the
intensity of the immune-positive reactions of
cytochrome C and Bax. These changes were ameliorated by concomitantly giving coenzyme Q10.
Conclusion: CoQ10 may ameliorate atorvastatin induced
skeletal muscle injury.
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Citation
Histology and Histopathology, Vol. 30, n.Âş 3 (2015)
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