Publication: Penning decoction ameliorated pyroptosis in mice with lipopolysaccharide-induced endometritis through inhibition of the TLR4/NF-κB/NLRP3 pathway
Authors
Chen Chen ; Yuqiong Yuan ; Zhihui Liu ; Qianru Zhou ; Jiani Shi
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Publisher
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Universidad de Murcia, Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-18-990
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info:eu-repo/semantics/article
Description
Abstract
Objectives. Endometritis, stemming from
bacterial infection, manifests as persistent inflammation
and may cause infertility. Penning decoction (PND) has
been approved for clinical treatment of patients with
endometritis. However, the mechanism by which it
prevents endometritis remains unknown. This study
aimed to examine the impact of PND on lipopoly
saccharide (LPS)-induced endometritis and elucidate the
underlying mechanisms involved.
Methods. Firstly, ultra-performance liquid
chromatography-quadrupole-time-of-flight mass
spectrometry (UPLC-Q-TOF-MS) analysis, in which
both positive and negative ion modes were used to
identify the chemical compounds in PND, was
performed. The antipyroptotic effects of PND were
validated in LPS-induced endometritis mice.
Additionally, mouse endometrial epithelial cells
(MEECs) were used to explore the molecular
mechanism of PND in serum in vitro.
Results. A total of 145 chemical compounds,
including flavones, saponins, polysaccharides, alkaloids,
and glycosides, were identified in positive and negative
ion modes. The results showed that LPS could induce
pyroptosis in endometritis in vivo and in vitro. Treatment
with PND or serum containing PND could significantly
ameliorate LPS-induced pyroptosis by inhibiting the
activation of the TLR4/NF-κB/NLRP3 signaling
pathway.
Conclusion. Our results demonstrated that PND may
improve LPS-induced endometritis by inhibiting the
TLR4/NF-κB/NLRP3 pathway, which provides a
potentially effective drug for the clinical treatment of
endometritis.
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