Publication:
Region-specific response of central microglial cells to sciatic nerve demyelination through sensory and motor pathways

Simple item page
dc.contributor.authorWu, Shuang
dc.contributor.authorSu, Yuxin
dc.contributor.authorWang, Yuqing
dc.contributor.authorWang, Jia
dc.contributor.authorXu, Dongsheng
dc.contributor.authorLiu, Yihan
dc.contributor.authorYang, Kunwu
dc.contributor.authorGao, Junhong
dc.contributor.authorCui, Jingjing
dc.date.accessioned2024-05-22T08:42:59Z
dc.date.available2024-05-22T08:42:59Z
dc.date.issued2024
dc.description.abstractPeripheral nerve injury can cause changes in microglial cells on the spinal dorsal and ventral horns. This region-specific response implies that central microglial cells could be activated through both sensory and motor pathways. In order to further determine how peripheral nerve injury activates central microglial cells through neural pathways, the sciatic nerve was selected as the target for neural tract tracing and demyelination. Firstly, we used cholera toxin subunit B (CTB) to map the central sensory and motor territories of the sciatic nerve. Secondly, we applied lysophosphatidylcholine to establish the model of sciatic nerve demyelination and examined the distribution of activated microglial cells via immunofluorescence with ionized calcium-binding adapter molecule 1. It was shown that CTB labeling included the transganglionically labeled sensory afferents and retrogradely labeled somata of motor neurons along the sensory and motor pathways of the sciatic nerve ipsilateral to the injection, in which sensory afferents terminated on the gracile nucleus, Clarke’s nucleus, and spinal dorsal horn, while motor neurons located on the spinal ventral horn. Consistently, after sciatic nerve demyelination, the activated microglial cells were observed in the same territories as CTB-labeling, showing shortened processes and enlarged cell bodies. These results support the idea that central microglia might be activated by signals from the demyelinated sciatic nerve through both sensory and motor pathways.es
dc.formatapplication/pdfes
dc.format.extent11es
dc.identifier.citationHistology and Histopathology Vol. 39, nÂş6 (2024)
dc.identifier.doihttps://doi.org/10.14670/HH-18-681
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/141759
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiaciĂłn externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPeripheral nerve injuryes
dc.subjectDemyelinationes
dc.subjectMicroglial cellses
dc.subjectSciatic nervees
dc.subjectLysophosphatidylcholinees
dc.subjectCholera toxin subunit Bes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - PatologĂ­a. Medicina clĂ­nica. OncologĂ­aes
dc.titleRegion-specific response of central microglial cells to sciatic nerve demyelination through sensory and motor pathwayses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Wu-39-771-781-2024.pdf
Size:
5.98 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Histology and histopathology Vol.39, nÂş6 (2024)