Publication: Monitoring of B cell in kidney transplantation: development of a novel clusters analysis and role of transitional B cells in transplant outcome
Authors
Alfaro, Rafael ; González Martínez, Gema ; Jiménez Coll, Víctor ; Martínez Banaclocha, Helios ; Galián, José Antonio ; Botella, Carmen ; Peña Moral, Jesús de la ; Moya Quiles, María Rosa ; Campillo, José Antonio ; Minguela, Alfredo ; Llorente, Santiago ; Muro, Manuel ; Legaz Pérez, Isabel
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Publisher
MDPI
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DOI
https://doi.org/10.3390/diagnostics11040641
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info:eu-repo/semantics/article
Description
© 2021 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Diagnostics. To access the final edited and published work see https://doi.org/10.3390/diagnostics11040641
Abstract
Background: B lymphocytes (BL) seem to play an important role in transplantation, although the and role of different subpopulations in monitoring and outcome is not clear. Our aim was to monitoring immunological profiles based on BL subpopulations in kidney recipients (KR) with the risk of acute rejection (AR). Methods: Monitoring of BL subpopulations was performed by flow cytometry in PBLs before transplantation and three and six months after transplantation (PTX). We used two methodological approaches, a traditional analysis, and a novel cluster analysis, to determine the association between BL subpopulations, AR incidence, and graft function. Results: After three months of PTX, KRs with a B phenotype enriched in transitional BL and plasmablasts had better kidney function and lower AR incidence. KRs with decreased transitional BL and plasmablasts were associated with lower kidney function and higher AR PTX. KRs that had an increase in transitional BL PTX had a better clinical outcome. The increase in transitory BL during PTX was also associated with an increase in Tregs. Indeed, KRs receiving thymoglobulin as induction therapy showed a slight decrease in the relative frequency of naive BLs after three months of PTX. Conclusion: The monitoring of BL subpopulations may serve as a non-invasive tool to improve immunological follow-up of patients after kidney transplantation. However, further studies are needed to confirm the obtained results, define cut-off values, and standardize more optimal and even custom/customized protocols.
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Citation
Diagnostics 2021, 11(4), 641
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