Publication: The effect of immunosuppressive therapy
on renal cell apoptosis in native rat kidneys
Authors
Kędzierska, Karolina ; Sporniak-Tutak, Katarzyna ; Kolasa, Agnieszka ; Domański, Leszek ; Domański, Maciej ; Sindrewicz, Krzysztof ; Smektała, Tomasz ; Bober, Joanna ; Safranow, Krzysztof ; Osękowska, Bogumiła ; Kabat-Koperska, Joanna ; Baranowska-Bosiacka, Irena ; Parafiniuk, Mirosław ; Urasińska, Elżbieta ; Ciechanowski, Kazimierz
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-30.105
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info:eu-repo/semantics/article
Description
Abstract
Aim: To analyse the impact of the most
commonly used immunosuppressive drugs on the
occurrence of apoptosis in the native kidneys of Wistar
rats. Method: The study involved 36 rats. The animals
grouped according to the immunosuppressive regimen
used (tacrolimus, mycophenolate mofetil, cyclosporine
A, rapamycin and prednisone). The rats in all study
groups were treated with a 3-drug protocol for 6 months.
The medication dose was adjusted based on available
literature data. No drugs were administered to the
control group. The rats were then killed. Autopsies of all
animals were performed and the kidneys were isolated
for histopathology (HE + PAS). To assess cell apoptosis
the TUNEL reaction was performed. Blood trough levels
of immunosuppressive drugs as well as the parameters of
peripheral blood were determined. Results: 1. In rats
treated with cyclosporine A distal nephron tubules were
characterised by more pronounced apoptosis. 2. In
tacrolimus-treated rats a lower intensity of apoptosis was
found in the distal tubules. 3. In rapamycin-treated rats
the apoptosis was inhibited both in the distal and
proximal nephron tubules. 4. In MMF treated rats
intense apoptosis was observed in the proximal nephron
tubules. 5. There were no significant changes in renal
histopathology (HE + PAS). Conclusions: The apoptosis
in nephron tubules caused by immunosuppressive
therapy is not accompanied by any histopathological
changes (eg fibrosis, inflammation, tubular atrophy,
vacuolation of the tubular cells) in light microscopy
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