A histidine kinase and a response regulator provide phage resistance to Marinomonas mediterranea via CRISPR-Cas regulation

Lucas-Elio, Patricia; Molina-Quintero, Luisa Raquel; Xu, Hengyi; Sánchez-Amat, Antonio

Publication:
A histidine kinase and a response regulator provide phage resistance to Marinomonas mediterranea via CRISPR-Cas regulation

dc.contributor.author	Lucas-Elio, Patricia
dc.contributor.author	Molina-Quintero, Luisa Raquel
dc.contributor.author	Xu, Hengyi
dc.contributor.author	Sánchez-Amat, Antonio
dc.contributor.department	Genética y Microbiología
dc.date.accessioned	2025-01-14T13:16:17Z
dc.date.available	2025-01-14T13:16:17Z
dc.date.issued	2021-10-18
dc.description	© The Author(s) 2021. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Scientific Reports. To access the final edited and published work see https://doi.org/10.1038/s41598-021-99740-9
dc.description.abstract	CRISPR-Cas systems are used by many prokaryotes to defend against invading genetic elements. In many cases, more than one CRISPR-Cas system co-exist in the same cell. Marinomonas mediterranea MMB-1 possesses two CRISPR-Cas systems, of type I-F and III-B respectively, which collaborate in phage resistance raising questions on how their expression is regulated. This study shows that the expression of both systems is controlled by the histidine kinase PpoS and a response regulator, PpoR, identified and cloned in this study. These proteins show similarity to the global regulators BarA/UvrY. In addition, homologues to the sRNAs CsrB and CsrC and the gene coding for the post-transcriptional repressor CsrA have been also identified indicating the conservation of the elements of the BarA/UvrY regulatory cascade in M. mediterranea. RNA-Seq analyses have revealed that all these genetics elements are regulated by PpoS/R supporting their participation in the regulatory cascade. The regulation by PpoS and PpoR of the CRISPR-Cas systems plays a role in phage defense since mutants in these proteins show an increase in phage sensitivity.	es
dc.format	application/pdf	es
dc.format.extent	13	es
dc.identifier.citation	Scientific Reports (2021) 11:20564
dc.identifier.doi	https://doi.org/10.1038/s41598-021-99740-9
dc.identifier.issn	Electronic: 2045-2322
dc.identifier.uri	http://hdl.handle.net/10201/148423
dc.language	eng	es
dc.publisher	Nature Research	es
dc.relation	Diversidad funcional de sistemas CRISPR-Cas. Papel de los sistemas con RT-Cas1 en la defensa frente a fagos. Ministerio de Economía, Industria y Competitividad (BFU2017-85464) Identificación de elementos genéticos infectivos diana de sistemas CRISPR-Cas. Fundación Séneca, Comunidad Autónoma de la Región de Murcia (20883/PI/18). The analysis of RNAseq data by Hengyi Xu was supported by NIH grants R01 GM37949 and R35 GM136216 to Alan M. Lambowitz.	es
dc.relation.publisherversion	https://www.nature.com/articles/s41598-021-99740-9
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Marinomonas mediterranea	es
dc.subject	CRISPR-Cas	es
dc.subject	Bacteriofago	es
dc.subject	Regulación	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología	es
dc.title	A histidine kinase and a response regulator provide phage resistance to Marinomonas mediterranea via CRISPR-Cas regulation	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es