Publication: The use of fluorescent dextrans as a marker of sarcolemmal injury
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Date
1994
Authors
Carter, G.T. ; Kikuchi, N. ; Horasek, S.J. ; Walsh, S.A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We investigated the use of intravenously
injected fluorescent dextran molecules (FDx) as a
histological marker of sarcolemmal injury. Using
fluorescent microscopy, uptake of FDx (average MW 10
kD) was assessed in sections of quadriceps muscles from
three models: l ) normal (C57BLlIOSnJ) mice. 2) normal
mice run downhill (0, 3, and 7 days post exercise), and
3) non-exercised mdx (dystrophin-deficient) mice. These
were compared to serial sections stained with
hematoxylin and eosin (H&E). In control muscles.
strong fluorescence was seen between fibers (intercellular).
Intracellular FDx was observed within cells of
the quadriceps from normal mice run downhill at days 0
and 3 post exercise, but not at day 7. On H&E staining.
muscle pathology was not observed until day 3, with
regeneration by day 7. Intracellular FDx was also
observed within mdx muscles, particularly in fibers that
appeared pre-necrotic on H&E stained sections. FDx
appears to be useful as a histological marker of changes
in sarcolemmal integrity associated with muscle injury
from eccentric exercise or muscle disease.
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