Publication: Molecular evidence of tissue remodeling in an animal model of heart failure
Authors
Silva Nascimento, Lais ; Martins Tedesco, Larissa ; Silva Araujo, Natiele ; Marinho Priviero, Fernanda Bruschi ; Claudino, Mario Angelo ; Gonçalves Priolli, Denise ; Rocha, Thalita
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-128
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info:eu-repo/semantics/article
Description
Abstract
Heart failure (HF) is the final common
pathway of many cardiovascular diseases.
Metalloproteinases and their inhibitors, such as MMP9
and TIMP-1, assist in maintaining the extracellular
matrix, leading to tissue remodeling observed after HF.
Previous studies have shown that L-Arginine (LA)
appears to have beneficial effects for the treatment of
HF, contributing to vasodilation, the reestablishment of
the endothelial function and an increase in muscle
contractile force. This study analyzed heart tissue
remodeling in an animal model of HF induced by
aortocaval fistula (ACF) and submitted to LA treatment.
After 4 weeks of ACF, animals were treated with LA for
4 weeks (SHAM-LA, HF-LA) or for 8-12 weeks with
saline (SHAM, HF8, HF12). Rats were euthanized and
the hearts removed for histological processing. The
samples were stained with Hematoxylin-Eosin (HE),
Masson’s Thichome (MT), or submitted to
immunohistochemistry (IHC) for MMP9 and TIMP-1.
Light microscopy analysis showed cardiac striated
muscle without fibrosis in all experimental groups.
Immunostaining of MMP9 and TIMP-1 were positive
for all experimental groups. LA administration
significatively reduced MMP9 content after HF. These
data indicate molecular changes in metalloproteinases
expression prior to tissue remodeling and point out LA
as an adjuvant therapy to pharmacological treatment of
patients with HF.
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Citation
Histology and Histopathology Vol. 34, nÂş 12 (2019)
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