Publication: Application of in vivo cryotechnique to the examination of cells and tissues in living animal organs
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Date
2006
Authors
Terada, N. ; Ohno, N. ; Li, Z. ; Fuji, Y. ; Baba, T. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
When all biological materials in cells and
tissues of living animal organs are quickly and promptly
frozen, immunolocalization of their components and
structural features in situ is necessary to understand their
in vivo functioning states. However, these direct
morphological analyses were difficult to achieve by
conventional chemical fixation methods during the last
century. A new cryofixation method, named the “in vivo
cryotechnique”, in which the normal blood circulation in
living animals is always retained at the moment of
freezing, has become a powerful tool to visualize the real
native morphology of cells and tissues with functional
meaning. The “in vivo cryotechnique” can usually be
combined with a wide range of subsequent preparation
techniques, and can thereby enable us to perform various
direct analyses on biological samples, reflecting the
physiological functions of living animal organs
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Citation
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