Publication: Effects of hypothyroidism on anti-mullerian hormone expression in the prepubertal rat testis
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Date
2008
Authors
Chamindrani Mendis-Handagama, S.M.L. ; Siril Ariyaratne, H.B.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Differentiation of adult Leydig cells (ALC)
in the prepubertal rat testis is stimulated by thyroid
hormone (Thy) and inhibited by the Anti-Mullerian
Hormone (AMH) produced by the immature Sertoli cell
(SC). As Thy induces SC maturation in the prepubertal
rat testis, we hypothesized that Thy stimulation of ALC
differentiation is mediated via inhibition of AMH
production by the SC with their maturation. If this
hypothesis is true, AMH production by the prepubertal
Sertoli cells in hypothyroid rats should not decline
immediately after birth as in euthyroid rats, but should
be maintained throughout the hypothyroid period at a
similar or higher level to that of day 1 rats. This concept
was tested using control rats of postnatal days (pd) 1, 7
and 14 and hypothyroid (fed 0.1% propyl thiouracil/PTU
to lactating mothers) rats of pd7 and pd14. Presence of
AMH in SC was examined by immunocytochemistry for
AMH. Results demonstrated that testes of pd1 rats had
intense AMH positive labeling exclusively in cytoplasm
of SC. In testes of pd7 and pd14 control and PTU rats, a
positive but weak labeling was also observed in
cytoplasm of some SC; Germ cells and testicular
interstitial cells were negative for AMH at all tested ages
in both experimental groups. These findings suggest that AMH production by the prepubertal SC is independent
of Sertoli cell maturation and not regulated by Thy.
Therefore, Thy regulation of ALC differentiation in the
prepubertal rat testis is unlikely to be mediated via
inhibition of AMH produced by the SC with their
maturation.
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