Publication: Expression and significance of cell immunohistochemical markers (HHF-35, CD-31, Bcl-2,
P-53 and apopDETEC®) in hypertrophic cardiomyopathy
Authors
Martínez Díaz, F. ; Bernal-Gilar, M. ; Gómez Zapata., Maximiliano ; Luna Maldonado, Aurelio
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
There are several hypotheses concerning the
pathogenesis of hypertrophic cardiomyopathy (genetic,
ischaemic, immune, inflammatory and apoptosis
induction). We have studied three types of cardiomyopathy
in order to observe the expression and assess
the significance of different immunohistochemical
markers (muscular actin, CD-31, proliferation cell
nuclear antigen -PCNA-, Ki-67, and markers related
with programmed cell death, bcl-2, p-53 and
apopDETEC®).
We studied different microscopic (haematoxylineosin
and Masson’s thrichrome) and immunohistochemical
parameters (streptavidin-biotin-peroxidase
and “in situ” hybridisation) of forty cases: ten each of
hypertensive hypertrophic cardiomyopathy, essential
hypertrophic cardiomyopathy, hypertrophic
cardiomyopathy in patients treated with chemotherapy
and morphologically “normal” hearts.
Our findings point to an absence of structural marker
expression (actin and CD-31) in cases of hypoxic
damage. The distribution and intensity of apoptosis
markers, a seen by “in situ” hybridisation were irregular,
and the rest of the markers studied showed negative
results, with the exception of acridin orange (a marker of
hypoxic damage).
In our opinion, the above immunohistochemical markers, especially actin and CD-31, could be used for
differentiating hypoxic lesions in these three types of
cardiomyopathy. Moreover, it is difficult to know the
significance of the apoptosis markers, because the
autolysis process produces cross reactions with false
positive results. We think that there is a need for new
studies on DNA breakdown processes during the postmortem
interval. To avoid autolysis problems the postmortem
material needs to be as fresh as possible.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.