Publication: Expression of certain proteins in
the subfornical organ and cerebrospinal
fluid of spontaneously hypertensive rats
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Date
2007
Authors
Gonzalez-Marrero, I. ; Carmona-Calero, E. ; Fernández-Rodríguez, P. ; Pérez-González, H. ; Ormazabal-Ramos, C. ; Castañeyra-Ruíz, L. ; Perez-Garcia, C.G. ; Martínez de la Peña y Valenzuela, Isabel ; Castañeyra-Ruiz, A. ; Castañeyra Perdomo, A. ; Ferres Torres, R.
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The objective of this study was to analyze
the proteins in the cerebrospinal fluid of spontaneously
hypertensive rats and to study their possible role in the
relationship between hydrocephalus, arterial
hypertension and variations in the subfornical organ.
Brains and cerebrospinal fluid from control Wistar-
Kyoto rats and spontaneously hypertensive rats
sacrificed with chloral hydrate were used. Cerebrospinal
fluid and extract of subfornical organ were processed by
protein electrophoresis. Antisera against protein bands of
141, 117 and 48 kDa and Concanavalin A were used for
immunohistochemical and western blot study of the
subfornical organ, adjacent circumventricular structures
and cerebrospinal fluid. Ventricular dilation in the
spontaneously hypertensive rats and the presence of
quite a lot of protein bands in the cerebrospinal fluid of
the hypertensive rats, which were either not observed or
scarcely present in the cerebrospinal fluid of the Wistar-
Kyoto rats, were confirmed. The subfornical organ, third
ventricle ependyma and choroideus plexus showed
immunoreactive material for antibodies against 141kDa,
117 and 48 kDa proteins band (anti-B1, anti-B2 and anti-
B3). The larger amount of the immunoreactive material
was found in the subfornical organ of the spontaneously
hypertensive rat. Our results and the alterations observed
by other authors in the subfornical organ in
hydrocephalic and hypertensive rats support the
possibility that this circumventricular organ, some
proteins of the cerebrospinal fluid and ventricular
dilation could be connected with the physiopathology of
this type of hypertension.
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