Publication:
Testicular cryopreservation: From technical aspects to practical applications

dc.contributor.authorPereira, Ana Glória
dc.contributor.authorMatos, Tayná Moura
dc.contributor.authorAlbuquerque, Joana Letícia Cottin de
dc.contributor.authorSilva, Andréia Maria da
dc.contributor.authorSilva, Alexandre Rodrigues
dc.contributor.departmentBiología Celular e Histologíaes
dc.date.accessioned2025-07-07T10:14:02Z
dc.date.available2025-07-07T10:14:02Z
dc.date.issued2025
dc.description.abstractTesticular cryopreservation has been highlighted as a promising alternative for preserving male fertility and can be applied to restore spermatogenesis in prepubertal individuals or cancer patients, preserve biologically valuable genotypes, and in studies on reproductive physiology or toxicity of various substances. This review presents an analysis of the technical aspects and applications of testicular cryopreservation, examining the contributions of important studies in this area and discussing the different factors that can impact the efficiency of the technique. Testicular fragments can be obtained from living or dead individuals, at any age and reproductive stage, through orchiectomy or biopsy. Among the methods used for processing, slow freezing and vitrification in open or closed systems stand out. However, factors such as species, age, medium used, cryoprotectants, and cryopreservation method can influence the viability of the testis after heating. To obtain sperm, the testes can be cultured in vitro or in vivo and the recovered gametes applied in assisted reproduction techniques. However, in some species, mainly wild animals and humans, this is still a limitation to be overcome.es
dc.formatapplication/pdfes
dc.format.extent12es
dc.identifier.doihttps://doi.org/10.14670/HH-18-869
dc.identifier.eisbnHistology and Histopathology Vol. 40, nº07 (2025)es
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/157041
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectSpermatogenesises
dc.subjectBiobankes
dc.subjectCryogenicses
dc.subjectFertility restorationes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleTesticular cryopreservation: From technical aspects to practical applicationses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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