Development of a time-resolved fluorometry based immunoassay for the determination of canine haptoglobin in various body fluids

Väisänen, Ville; Cerón Madrigal, José Joaquín; Parra Muñoz, María Dolores

Publication:
Development of a time-resolved fluorometry based immunoassay for the determination of canine haptoglobin in various body fluids

dc.contributor.author	Väisänen, Ville
dc.contributor.author	Cerón Madrigal, José Joaquín
dc.contributor.author	Parra Muñoz, María Dolores
dc.contributor.department	Ciencias Sociosanitarias
dc.date.accessioned	2025-01-22T08:03:39Z
dc.date.available	2025-01-22T08:03:39Z
dc.date.issued	2005
dc.description	© INRA, EDP Sciences 2005. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. This document is the Published version of a Published Work that appeared in final form in Veterinary Research. To access the final edited and published work see https://doi.org/10.1051/vetres:2004054	es
dc.description.abstract	A time-resolved immunofluorometric assay (TR-IFMA) was developed for the determination of haptoglobin (Hp) in canine serum. Haptoglobin was purified from canine acute phase serum by ammonium sulphate precipitation followed by gel filtration. This isolated dog Hp was used as the standard to calibrate the assay. Intra- and inter-assay coefficients of variation of the assay were, respectively, 5.7% and 16.6% at 0.51 mg/mL, 2.4% and 10.6% at 2.1 mg/mL and 10.5% and 11.9% at 32.5 mg/mL. The dilution of serum samples with high Hp concentrations resulted in linear regression equations with R2 of 0.99 and 0.97. A high correlation was found in serum Hp measurements by TR-IFMA and a commercial assay based on peroxidase activity of haemoglobin bound to haptoglobin (R2 = 0.96). The limit of detection for the TR-IFMA method was 0.002 µg/mL. The addition of fresh haemolysate to serum samples did not affect the haptoglobin concentration (P = 0.694). Statistical differences (P < 0.003) were found between healthy dogs and dogs with different pathological processes. In whole blood, Hp concentrations were much lower than in serum but closely related (R2 = 0.84) whereas saliva Hp concentrations were poorly related with serum concentrations (R2 =0.53). However, the concentration of Hp in saliva was significantly (P < 0.039) higher in dogs with pathological processes compared to healthy dogs. The assay sensitivity was adequate to also be applied to whole blood and saliva specimens.	es
dc.format	application/pdf	es
dc.format.extent	13	es
dc.identifier.citation	Vet. Res. 36 (2005) 117-129
dc.identifier.doi	https://doi.org/10.1051/vetres:2004054
dc.identifier.issn	Print: 0928-4249
dc.identifier.issn	Electronic: 1297-9716
dc.identifier.uri	http://hdl.handle.net/10201/148984
dc.language	eng	es
dc.publisher	INRA / EDP Sciences	es
dc.relation	Sin financiación externa a la Universidad	es
dc.relation.publisherversion	https://www.vetres.org/articles/vetres/abs/2005/01/V4041/V4041.html	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Canine	es
dc.subject	Haptoglobin	es
dc.subject	Time-resolved fluorometry	es
dc.subject	Whole blood	es
dc.subject	Saliva	es
dc.title	Development of a time-resolved fluorometry based immunoassay for the determination of canine haptoglobin in various body fluids	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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