Publication: Analysis of Epstein-Barr virus strains and variants in classical Hodgkin s lymphoma by laser microdissection
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Date
2008
Authors
García-Cosío, Mónica ; Santón, Almudena ; Martín, Paloma ; Reguero, María Eugenia ; Cristóbal, Eva ; Bellas, Carmen
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Epstein-Barr virus (EBV) seems to have an
etiological role in the pathogenesis of classical
Hodgkin’s lymphoma (cHL). Studies of whole tissue
DNA by polymerase-chain reaction (PCR) have shown a
considerable number of cHL cases with co-infections by
different EBV strains and variants, which apparently
contradict the clonality of EBV in cHL previously
demonstrated by Southern blot analysis. Due to the
paucity of HRS cells in HL tissues, studies on single cell
DNA are necessary to identify the specific cellular
location (HRS cells and/or bystander B lymphocytes) of
the EBV strains and variants present in tissue specimens.
In the current study, the presence of EBV was
determined by PCR of the 3’ end of the LMP-1 gene and
EBNA-3C gene in whole tissue and, consecutively, in
isolated cells from 26 cases of cHL: 10 HIV-positive and
16 sporadic cHL cases. EBV EBERs were present in all
but 2 sporadic cHL cases, which were used as negative
controls. At isolated cell level, EBNA-3C gene PCR was
more sensitive. Indeed, from the cHL cases in which
dual-infection was present, it was observed that, in most
of them, HRS cells were infected by type 1 virus, and B
lymphocytes were co-infected by both types, which
points towards EBV infection occurring early in cHL development. Moreover, the finding of 2 cases with
dual-infection in HRS may suggest that, in a small
percentage of cHL cases, HRS cells derive from
different neoplastic clones, or that HRS cells are
superinfected by other viral types after the establishment
of the neoplastic clone.
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