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The total electric charge and time of application of galvanic currents to macrophages can optimize the release of IL-1β with low cell death

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dc.contributor.authorPeñín Franc, Alejandro
dc.contributor.authorGarcía Vidal, José Antonio
dc.contributor.authorGómez, Ana Isabel
dc.contributor.authorEscolar Reina, Pilar
dc.contributor.authorMedina Mirapeix, Francesc
dc.contributor.authorPelegrín, Pablo
dc.contributor.departmentFisioterapia
dc.date.accessioned2025-01-20T13:04:01Z
dc.date.available2025-01-20T13:04:01Z
dc.date.issued2024-12-28
dc.description© The Author(s) 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published version of a Published Work that appeared in final form in Scientific Reports. To access the final edited and published work see https://doi.org/10.1038/s41598-024-81848-3es
dc.description.abstractGalvanic current has been emerging as a novel therapy to regenerate chronic tissue lesions, including musculoskeletal and dermatological lesions. Recently, the NLRP3 inflammasome and IL-1β release have been identified as a signaling pathway triggered upon galvanic current application. However, the parameters for the clinical application of galvanic current remain subjective to the experience of the facultative in charge. In this study we used an in vitro model of macrophage culture and application of different combinations of the parameters of galvanic current to study IL-1β production and cell death. Increasing electric charge of galvanic current induces the release of IL-1β, but electric charges equal or higher to 144 mC also increase cell death. The release of IL-1β have a substantial variation within different electric charge of galvanic currents, being increased by decreasing the current and increasing the time of current application. Within the range of current intensities studied, the most optimal protocol for maximizing IL-1β release without inducing cell death was identified at electric charges equal to or near 144 mC, applied over a total duration of approximately 25 s. Our findings lay the groundwork for future in vivo studies assessing different electric charge of galvanic current, with the aim of yielding clinically relevant outcomes.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.citationScientific Reports, 2024, Vol. 14 : 30871
dc.identifier.doihttps://doi.org/10.1038/s41598-024-81848-3
dc.identifier.urihttp://hdl.handle.net/10201/148844
dc.languageenges
dc.publisherNature Researches
dc.relationA.P-F. was supported by MVClinic and Prim. This work was supported by grants to P.P. from FEDER/Ministerio de Ciencia, Innovación y Universidades – Agencia Estatal de Investigación (grant PID2020-116709RB-I00), MCIN/AEI/https://doi.org/10.13039/501100011033 and European Union «Next Generation EU/PRTR» (grant CNS2022-135101 to PP), and Fundación Séneca (grant 21897/PI/22 to PP).es
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-024-81848-3es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPercutaneous needle electrolysises
dc.subjectGalvanic currentes
dc.subjectMacrophagees
dc.subjectInflammationes
dc.subjectIL 1βes
dc.subjectCell deathes
dc.titleThe total electric charge and time of application of galvanic currents to macrophages can optimize the release of IL-1β with low cell deathes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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