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Morphological and immunohistochemical evaluation of ganglion cysts. Cross-sectional study of 354 cases

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dc.contributor.authorO’Valle, Francisco
dc.contributor.authorHernández-Cortés, Pedro
dc.contributor.authorAneiros Fernández, José
dc.contributor.authorCaba Molina, Mercedes
dc.contributor.authorGómez-Morales, Mercedes
dc.contributor.authorCámara, Miguel
dc.contributor.authorPayá, Jorge A.
dc.contributor.authorAguilar, David
dc.contributor.authorDel Moral, Raimundo G.
dc.contributor.authorAneiros, Jose
dc.date.accessioned2019-03-29T08:48:42Z
dc.date.available2019-03-29T08:48:42Z
dc.date.issued2014
dc.description.abstractThe aim of this study was to characterize the morphology and immunophenotype of ganglion cysts (GCs) and explore their histogenetic origin. Material and Methods: A cross-sectional morphological and immunohistochemical study of 354 GCs used the following antibody panel: vimentin, specific actin, ß- actin, smooth-muscle actin, smoothelin, h-caldesmon, ß- catenin, desmin, calponin, podoplanin, keratins 5/6, Ecadherin, cyclooxygenase 2 (COX-2), lysozyme, CD10, CD31, CD33, CD34, CD68, Ki-67, and PCNA. Doubleblind semi-quantitative analyses were conducted to evaluate the immunopositivity on a 4-point scale. Samples from 10 synovial membranes and 10 scapholunate ligaments were compared. GCs showed a hyalinized wall with mesenchymal spindle cells and were intensely positive for vimentin, actins, h-caldesmon, calponin in all cases and for podoplanin in 53% of cases, suggesting features of early muscle differentiation, without ruling out a myofibroblastic origin. Focal cavity lining of nonsynovial flat or raised cells (CD34/CD31/CD10/Ecadherin-negative and podoplanin-positive in 34% of cases) was detected in 93% of cases, showing differential expression with synovial membrane and scapholunate ligament cells. Nuclear positivity for proliferative markers was observed in GC wall cells (258.1±255; 1019.3±316 positive cells/mm2, Ki-67 and PCNA, respectively) but positivity for these markers was significantly lower (p<0.001 Mann Whitney U-test) in scapholunate ligament samples. Conclusion: In this first immunohistochemical study of GCs, focal cellular lining of the cavity was observed in almost all cases, and the immunophenotype was identical to that of GC wall cells. These cells are immunohistochemically different from synoviocytes and scapholunate ligament cells and show characteristics of myofibroblasts or mesenchymal cells undergoing early muscle differentiation.es
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dc.format.extent7es
dc.identifier.eisbnHistology and Histopathology, vol. 29, nº 5, (2014)es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/68699
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectGanglion cystes
dc.subjectSoft tissuees
dc.subjectMultifunctional mesenchymal cellses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleMorphological and immunohistochemical evaluation of ganglion cysts. Cross-sectional study of 354 caseses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.29, nº 5 (2014)