Publication: Immunostaining of proinflammatory cytokines in renal cortex and medulla of rats exposed to gold nanoparticles
Authors
Khan, Haseeb A. ; Ibrahim, Khalid E. ; Khan, Ayaat ; Alrokayan, Salman H. ; Alhomida, Abdullah S.
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-825
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info:eu-repo/semantics/article
Description
Abstract
Recently, gold nanoparticles (GNPs) have
shown promising applications in targeted drug delivery
and contrast imaging. Although in vitro cytotoxicity of
GNPs has been thoroughly studied, there are limited data
on in vivo toxicity of GNPs. In this study, we evaluated
the effects of intraperitoneally injected 10 nm and 50 nm
GNPs (5 µg/animal) on the expression of proinflammatory cytokines (IL-1β, IL-6 and TNF-α) on day 1 and
day 5, post-exposure. The results of immunohistochemistry showed that both 10 nm and 50 nm GNPs
induced an acute phase expression of proinflammatory
cytokines in renal cortex and medulla. This
proinflammatory response was comparatively more
intense in renal medulla than cortex. All the three
cytokines were undetectable in control cortex and
medulla. In conclusion, both 10 nm and 50 nm GNPs
caused an acute phase induction of proinflammatory
cytokines in cortex and medulla of rat kidneys. An
intense immunostaining of proinflammatory cytokines in
renal medulla warrants further studies to evaluate the
nephrotoxicity of GNPs to validate the safe application
of GNPs for contrast imaging in renal insufficiency.
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Citation
Histology and Histopathology, Vol.32, nº6, (2017)
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