Effect of superovulation treatment on oocyte´s DNA methylation.

Lopes, J.S.; Ivanova, E.; Ruiz, S.; Andrews, S.; Kelsey, G.; Coy, P.

Publication:
Effect of superovulation treatment on oocyte´s DNA methylation.

dc.contributor.author	Lopes, J.S.
dc.contributor.author	Ivanova, E.
dc.contributor.author	Ruiz, S.
dc.contributor.author	Andrews, S.
dc.contributor.author	Kelsey, G.
dc.contributor.author	Coy, P.
dc.contributor.department	Fisiología
dc.contributor.department	Epigenetics Programme. Babraham Institute. Cambridge, U.K.
dc.contributor.department	Bioinformatics Group. Babraham Institute. Cambridge, U.K.
dc.contributor.department	Centre for Trophoblast Research. Cambridge University. U.K.
dc.date.accessioned	2024-01-29T08:44:59Z
dc.date.available	2024-01-29T08:44:59Z
dc.date.issued	2022-12-18
dc.description	© 2022. The authors. This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the accepted version of a published work that appeared in final form in International Journal of Molecular Sciences	es
dc.description.abstract	Controlled ovarian stimulation is a necessary step in some assisted reproductive procedures allowing a higher collection of female gametes. However, consequences of this stimulation for the gamete or the offspring have been shown in several mammals. Most studies used comparisons between oocytes from different donors, which may contribute to different responses. In this work, we use the bovine model in which each animal serves as its own control. DNA methylation profiles were obtained by single-cell whole-genome bisulfite sequencing of oocytes from pre-ovulatory unstimulated follicles compared to oocytes from stimulated follicles. Results show that the global percentage of methylation was similar between groups, but the percentage of methylation was lower for non-stimulated oocytes in the imprinted genes APEG3, MEG3, and MEG9 and higher in TSSC4 when compared to stimulated oocytes. Differences were also found in CGI of imprinted genes: higher methylation was found among non-stimulated oocytes in MEST (PEG1), IGF2R, GNAS (SCG6), KvDMR1 ICR UMD, and IGF2. In another region around IGF2, the methylation percentage was lower for non-stimulated oocytes when compared to stimulated oocytes. Data drawn from this study might help to understand the molecular reasons for the appearance of certain syndromes in assisted reproductive technologies-derived offspring.	es
dc.format	application/pdf	es
dc.format.extent	19	es
dc.identifier.citation	International Journal of Molecular Sciences 2022, 23(24), 16158
dc.identifier.doi	https://doi.org/10.3390/ijms232416158
dc.identifier.issn	1661-6596
dc.identifier.uri	http://hdl.handle.net/10201/137902
dc.language	eng	es
dc.publisher	MDPI	es
dc.relation	Ambito Nacional. Entidad financiadora: Proyectos de I+D+I, del Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los retos de la sociedad. Nombre: Obtención de animales sanos mediante técnicas de reproducción asistida basadas en condiciones fisiológicas. Ref. AGL2015-66341-R. - Ambito Europeo. Entidad financiadora: European Comission. Research Executive Agency (REA). European Joint Doctorate in Biology and Technology of Reproductive Health. Marie Sklodowska-Curie Innovative Training Network. Ref. REP-BIOTECH 675526. - Ambito Nacional. Entidad financiadora: Agencia de Ciencia y Tecnología de la Región de Murcia. Fundación Séneca Ayudas a los Grupos y Unidades de Excelencia Científica de la Región de Murcia. Nombre: From assisted reproductive technologies onwards: their impact on preimplantation epigenetic reprogramming and their use in the generation of CRISPR-Cas9 transgenic animals. Ref. 20040/GERM/16.	es
dc.relation.publisherversion	https://www.mdpi.com/1422-0067/23/24/16158	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Oocyte	es
dc.subject	DNA methylation	es
dc.subject	Ovarian stimulation	es
dc.subject	ART	es
dc.subject	Epigenetics	es
dc.title	Effect of superovulation treatment on oocyte´s DNA methylation.	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es