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Redox profiling of preovulatory follicular fluid in the donkey is species-specific, and contributes to modulate sperm function

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dc.contributor.authorCatalán, Jaime
dc.contributor.authorPadilla, Lorena
dc.contributor.authorMaside, Carolina
dc.contributor.authorYánez-Ortiz, Iván
dc.contributor.authorTvarijonaviciute, Asta
dc.contributor.authorBarranco Cascales, Isabel
dc.contributor.authorBonet, Sergi
dc.contributor.authorMiró, Jordi
dc.contributor.authorYeste, Marc
dc.contributor.authorMartínez Hernández, Jesús
dc.contributor.departmentMedicina y Cirugía Animal
dc.contributor.otherFacultad de Veterinaria
dc.date.accessioned2025-10-17T07:35:01Z
dc.date.available2025-10-17T07:35:01Z
dc.date.copyright© The Author(s) 2025
dc.date.issued2025-02-23
dc.description.abstractThe follicular fluid (FF) is crucial for providing oocytes with an ideal environment that promotes their development and maturation. Not only does this fluid supply nutrients and hormones, but also other components that protect both follicular cells and the oocyte itself from potential harmful factors, such as those inducing oxidative stress (OS). The FF has also been suggested to have beneficial effects on sperm when they reach the oviduct. The objectives of this study were to describe the presence and activity levels of redox biomarkers in the preovulatory follicular fluid (PFF), and to analyze the impact of PFF on the motility, viability and reactive oxygen species (ROS) levels of donkey sperm. For this purpose, ten PFF samples obtained from Catalan jennies and nine ejaculates collected from Catalan donkeys were used. Redox biomarkers -including enzymatic and non-enzymatic antioxidants, and oxidative biomarkers- were analyzed in jennies' PFF. After collection, each semen sample was split into two aliquots of equal volume. The two aliquots were centrifuged to remove the seminal plasma; one pellet was resuspended in Tris Buffered Medium (TBM), and the other was resuspended in TBM supplemented with 20% PFF. Motility parameters (CASA) and other semen quality biomarkers (flow cytometry) were assessed after 0, 60 and 120 min of incubation at 38 °C. Exposure of donkey sperm to PFF reduced intracellular ROS levels and helped maintain sperm motility and viability. These findings suggest that, in the oviduct, the PFF components protect donkey sperm from oxidative stress. Furthermore, knowing better the composition of donkey PFF in terms of antioxidant biomarkers may be used to improve the formulation of media for oocyte maturation and fertilization in this and other species.
dc.formatapplication/pdf
dc.format.extent14
dc.identifier.citationScientific Reports (2025) 15:6522
dc.identifier.doihttps://doi.org/10.1038/s41598-025-91422-0
dc.identifier.eissn2045-2322
dc.identifier.urihttp://hdl.handle.net/10201/167349
dc.languageeng
dc.publisherNature Research
dc.relationJ.C. was funded by the Spanish Ministry of Science, Innovation and Universities (MCIN/AEI/10.13039/501100011033) and the European Union NextGenerationEU/PRTR (Juan de la Cierva Scheme: JDC2022-049684-I). I.B. was funded by the Spanish Ministry of Science and Innovation (MCIN/AEI/10.13039/501100011033) and the European Union NextgenerationEU/PRTR (Ramón y Cajal Scheme: RYC2021-034546-I), and J.M. was funded by the European Union-Next Generation EU Funds (University of Murcia, Margarita Salas Scheme 181/MSJD/22). The authors also acknowledge the support from the Ministry of Science and Innovation, Spain (Grant: PID2020-113320RBI00); the Catalan Agency for the Management of University and Research Grants, Regional Government of Catalonia, Spain (Grant: 2017-SGR-1229 and 2021-SGR-00900); and the Catalan Institution for Research and Advanced Studies, Spain (ICREA).
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-025-91422-0
dc.rightsAttribution-NonCommercial-NoDerivates 4.0 Internacional
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAntioxidants
dc.subjectDonkey
dc.subjectFollicular fluid
dc.subjectOxidative stress
dc.subjectSperm
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleRedox profiling of preovulatory follicular fluid in the donkey is species-specific, and contributes to modulate sperm function
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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relation.isAuthorOfPublication3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
relation.isAuthorOfPublicatione442a579-c2af-4fdd-8e39-00e5581604cb
relation.isAuthorOfPublication.latestForDiscovery51159b46-4514-4a54-9cc7-dea538fc1efa
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