Publication: In vivo toxicity of the ribosome- inactivating lectin ebulin f in elderly mice
Authors
Garrosa, Manuel ; Jiménez, Pilar ; Córdoba Díaz, Damián ; García Recio, Verónica ; Gayoso, Sara ; Rojo, María Ángeles ; Gayoso, Manuel J. ; Girbés, Tomás
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-992
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info:eu-repo/semantics/article
Description
Abstract
Ebulin f is a ribosome-inactivating protein
(RIP) present in green fruits of the dwarf elder
(Sambucus ebulus L). Since dwarf elder fruits are used
for food and as a medicine, we assessed the study of
toxicological effects and safety of ebulin f in elderly
mice, comparing these results with those reported in
young animals and with other RIPs. Female Swiss mice
aged 6 and 12 months of age were intraperitoneally
injected with a single dose from 1.4 to 4.5 mg/kg ebulin
f. Heart, stomach, intestines, lung, kidney, liver, spleen,
pancreas, adrenal gland, uterus, ovary and brain were
studied. Histology analysis was carried out by staining
with hematoxylin and eosin and Masson’s trichrome
observed with a light microscope, or apoptosis detection
by TUNEL method observed with a confocal laser
microscope. Treated animals injected with the lower
dose could recover their weights, but after 14 days half
of them died. The higher dose caused a progressive loss
of body weight leading to death. In the animals of the
experimental groups it was found atrophy of
Lieberkühn’s crypts, pneumonia, nephronal
degeneration, myocardial atrophy, centrolobular hepatic
necrosis, splenic white pulp necrosis foci and increased
rate of apoptosis in the intestines and liver, in which
apoptoses were mainly located in the vicinity of the
lobular central vein. We conclude that ebulin f affects
vital organs in elderly mice.
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Citation
Histology and Histopathology, Vol.33, nº9, (2018)
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