Publication: The density and distribution of follicular dendritic cells
in rat popliteal lyrnph nodes: a decrease in their density
and a change in their distribution after stirnulation
Authors
Sato, H. ; Dobashi, Michio
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
To determine whether follicular dendritic
cells (FDCs) increase after stimulation, and also to
confirm the widely accepted finding that the density of
FDCs in the light zone is higher than that in the dark
zone, we examined the density of FDCs in lymph
follicles of rat popliteal lymph nodes. Rats aged five
weeks were stimulated by injection of sheep
erythrocytes, and then examined 10 days later.
Unstimulated rats were also examined. After embedding
in paraffin, the removed lymph nodes were
immunostained with anti-S-100 protein antibody as a
marker of rat FDCs. The density of FDCs was
determined by measuring the area of the lymph follicle
and counting the number of S-100 protein-positive cells
within it. The density of FDCs in the lymph follicles of
stimulated rats was found to be significantly lower than
in 5-week-old and unstimulated rats. The density of
FDCs in the light zone was similar to that in the dark
zone when germinal center bordering cells (GCBCs),
distributed at the border between the dark zone and the
adjacent corona, were counted as FDCs. We conclude
that the density of FDCs in lymph follicles decreases
after stimulation, and suggest that FDCs have no or only
very slight proliferative activity under normal
conditions. Investigators may need to consider GCBCs
in order to understand how FDCs differentiate.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.