Publication: Ultrastructural localisation of acid phosphatase in intestinal eosinophilic granule cells (EGC) of rainbow trout
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Date
1992
Authors
Powell, Mark D. ; Briand, Heather A. ; Wright, Glenda M. ; Burka, John F.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Enzyme cytochemistry was used to
investigate possible lysosome involvement in capsaicin
induced degranulation of the eosinophilic granule cell
(EGC) of the rainbow trout intestine. Three adult
rainbow trout (Oncorhynchus mykiss) were injected
intra 'k eritoneally with capsaicin in a saline vehicle (0.5 pg.g- body weight). Following a 2 hour period of
incubation, the fish were killed, and a mid portion of
the intestine was dissected and fixed in cold
glutaraldehyde buffered with sodium cacodylate.
Vibratome sections were incubated in either reaction
medium containing B-glycerophosphate and cerium
chloride in acetate buffer or substrate (Bglycerophosphate)
deficient control medium. Sections
were then refixed in osmium tetroxide and processed
for electron microscopy. Acid phosphatase was found
to be localised within lysosomes. The enzyme was not
found in the large cytoplasmic granules under normal
or capsaicin-stimulated conditions. EGCs which had
migrated to the lamina propria in response to the
capsaicin stimulation had a distinct multivesicular
granule morphology. These multivesicular granules did
not contain acid phosphatase suggesting that this form
of EGC degranulation is not a lysosomally mediated
event.
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