Publication: TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus
Authors
Andrzej Lipiec, Marcin ; Bem, Joanna ; Koziński, Kamil ; Chakraborty, Chaitali ; Urban-Ciećko, Joanna ; Zajkowski, Tomasz ; Dabrowski, Michał ; Mateusz Szewczyk, Łukasz ; Toval, Ángel ; Ferrán Bertone, José Luis ; Nagalsk, Andrzej ; Wiśniewska, Marta Barbara
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Facultad de Medicina
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Publisher
The company of Biologists Ltd.
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DOI
https://doi.org/10.1242/dev.190181
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info:eu-repo/semantics/article
Description
Abstract
Neuronal phenotypes are controlled by terminal selector transcription factors in invertebrates, but only a few examples of such regulators have been provided in vertebrates. We hypothesised that TCF7L2 regulates different stages of postmitotic differentiation in the thalamus, and functions as a thalamic terminal selector. To investigate this hypothesis, we used complete and conditional knockouts of Tcf7l2 inmice. The connectivity and clustering of neurons were disrupted in the thalamo-habenular region in Tcf7l2−/− embryos. The expression of subregional thalamic and habenular transcription factors was lost and region-specific cell migration and axon guidance genes were downregulated. In mice with a postnatal Tcf7l2 knockout, the induction of genes that confer thalamic terminal electrophysiological features was impaired. Many of these genes proved to be direct targets of TCF7L2. The role of TCF7L2 in terminal selection was functionally confirmed by impaired firing modes in thalamic neurons in the mutantmice. These data corroborate the existence of master regulators in the vertebrate brain that control stage-specific genetic programmes and regional subroutines, maintain regional transcriptional network during embryonic development, and induce terminal selection postnatally.
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Citation
Development (2020) 147, dev190181
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