Publication: Recombinant Vaccine for Canine Parvovirus in Dogs
Authors
López de Turiso, José Ángel ; Cortés, Elena ; Martínez, Concepción ; Ruiz de Ybáñez Carnero, María del Rocío ; Simarro, Isabel ; Vela, Carmen ; Casal, Ignacio
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Publisher
American Society for Microbiology
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DOI
https://doi.org/ 10.1128/JVI.66.5.2748-2753.1992
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info:eu-repo/semantics/article
Description
©1992,American Society for Microbiology. This manuscript version is made available under the CC-BY NC/ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
This document is the Published, version of a Published Work that appeared in final form in Journal of Virology. To access the final edited and published work see https://doi.org/ 10.1128/JVI.66.5.2748-2753.1992
Abstract
VP2 is the major component of canine parvovirus (CPV) capsids. The VP2-coding gene was engineered to be
expressed by a recombinant baculovirus under the control of the polyhedrin promoter. A transfer vector that
contains the lacZ gene under the control of the plO promoter was used in order to facilitate the selection of
recombinants. The expressed VP2 was found to be structurally and immunologically indistinguishable from
authentic VP2. The recombinant VP2 shows also the capability to self-assemble, forming viruslike particles
similar in size and appearance to CPV virions. These viruslike particles have been used to immunize dogs in
different doses and combinations of adjuvants, and the anti-CPV responses have been measured by
enzyme-linked immunosorbent assay, monolayer protection assays, and an assay for the inhibition of
hemagglutination. A dose of ca. 10 ,ug of VP2 was able to elicit a good protective response, higher than that
obtained with a commercially available, inactivated vaccine. The results indicate that these viruslike particles
can be used to protect dogs from CPV infection.
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