Publication: The thoracic sympathetic neurons of the chick, normal development and the effects of nerve growth factor
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Date
1986
Authors
Smet, Peter ; Rush, Robert A. ; Straznicky, Charles
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The generation and degeneration of
sympathetic neurons in the third thoracic ganglion (segment
19) of the chick were studied between embryonic days (E)
7-18 using 3H-Thymidine autoradiography and routine cell
counts. Cumulative radiolabelling experiments indicated
that few sympathetic neurons were generated on E6-7. 10%
of the sympathetic neurons were generated on E8 and a
further 20% on E9. The final 70% of neurons completed
the mitotic cycle between E10-12. Cell counts demonstrated
that the neuronal population increased from
10,166 + 423 (mean 2 SEM) to 22,291 ? 767 between
E8-10 and remained stable up to E14. The population
subsequently declined by 37%, to 14,157 + 831, by E18.
Pyknotic neurons were found at all stages of development,
but were most apparent between E7-15. The effects of
Nerve Cirowth Factor (NGF) on the number of both
surviving and pyknotic neurons in the ganglion were also
examined. E9 embryos treated with NGF from E5-8 showed
a 57% increase in the number of sympathetic neurons. This
increase therefore occurred prior to the decline in neuronal
number and was not accompanied by a decrease in the
number of visibly pyknotic neurons. It is therefore possible
that early NGF treatment increases the number of
sympathetic neurons through a mechanism other than the
attenuation of cell death.
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