Publication: Expression pattern of the endoplasmic reticulum stress protein GP96 in monophasic and chronic relapsing form of experimental autoimmune encephalomyelitis in rats
Authors
Jakovac, Hrvoje ; Grebić, Damir ; Barac-Latas, Vesna ; Mrakovčić-Šutić, Ines ; Radošević-Stašić, Biserka
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Gp96 is the endoplasmic reticulum (ER)-
resident molecular chaperone, which is involved in the
correction of unfolded proteins, in the activation of
proteasome-dependent ER-associated degradation of the
misfolded proteins, and in activation of the protein
translation that modulates polypeptide traffic into the
ER. Furthermore, owing to its peptide chaperone
capacity and ability to interact with professional antigenpresenting
cells, as well as with growth factors, integrins
and Toll-like receptors, it is also endowed with crucial
immunological functions acting as a “danger signal” to
the innate and adaptive immunity.
Considering these properties, in the present study the
tissue expression of gp96 was examined during the
monophasic and chronic relapsing form of experimental
autoimmune encephalomyelitis (CR-EAE), induced in
genetically susceptible DA rats by subcutaneous
injection of myelin basic protein (MBP) or bovine brain
homogenate in complete Freund's adjuvant (CFA).
Immunohistochemical analyses were done in periods of
attacks and remissions of EAE, and the results were
compared with findings in intact rats and those treated
only with CFA. The data revealed that the constitutive
gp96 expression, found in several neurons and glial cells
in the brain and spinal cord of intact animals,
significantly diminished during the attacks of CR-EAE.
On the contrary, the remission of disease was followed
by high upregulation of gp96, mainly in the
oligodendrocytes within the white matter, in the neurons
of the hippocampal area, as well as in the motoneurons
of lumbar spinal cord, suggesting that gp96 might be
involved in proteostasis and immune-related pathways
linked with the reparative processes in the CNS.
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Citation
Histology and histopathology, Vol. 28, n.º 1 (2013)
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