Publication: Isolation and characterization of FS01, a lytic bacteriophage infecting Salmonella typhimurium
| dc.contributor.author | Guillén-Vásquez, Abraham | |
| dc.contributor.author | Zumaeta, Kattya | |
| dc.contributor.author | Talledo, Miguel | |
| dc.contributor.department | Sin departamento asociado | |
| dc.date.accessioned | 2025-12-22T16:37:30Z | |
| dc.date.available | 2025-12-22T16:37:30Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Salmonella contamination represents a health and economic problem. This study isolated and characterized a lytic bacteriophage, FS01, active against S. typhimurium. FS01 showed a MOI of 1, anadsorption rate of 1.9x10-9 mL/min, and a growth curve with an eclipse period of 10 minutes, a latency period of 15 minutes, and a burst size of 10 virions per cell. It has an icosahedral capsid (64.4 nm) and a flexible tail (182.2 nm), suggestive of Siphoviridae family affiliation, with a narrow host range. It is thermally stable between 40–60 ºC for 30 minutes, viable within a pH range of 6–9, and sensitive to chloroform and UV light. FS01 inhibited bacterial growth starting at 3 hours. FS01 exhibits properties of a promising biocontrol agent for decontaminating food and surfaces. | en |
| dc.description.abstract | La contaminación por Salmonella representa un problema sanitario y económico. Este estudio aisló y caracterizó un bacteriófago lítico, FS01, activo contra S. typhimurium. FS01 mostró una MOI de 1, tasa de adsorción de 1,9x10-9 mL/min, y una curva de crecimiento con eclipse de 10 minutos, latencia de 15 minutos y tamaño de explosión de 10 viriones por célula. Posee cápside icosaédrica (64,4 nm) y cola flexible (182,2 nm), lo que sugiere pertenecer a la familia Siphoviridae, con estrecho rango de hospedero; es estable térmicamente entre 40–60 ºC por 30 minutos, viable entre pH 6–9, sensible a cloroformo y luz UV. FS01 inhibió el crecimiento bacteriano desde las 3 horas. FS01 presenta propiedades de un agente biocontrolador prometedor para descontaminar alimentos y superficies. | es |
| dc.format | application/pdf | |
| dc.format.extent | 11 | |
| dc.identifier.citation | Anales de Biología, Vol. 47 (2025) 49-58 | |
| dc.identifier.eissn | 1989-2128 | |
| dc.identifier.issn | 1138-3399 | |
| dc.identifier.uri | http://hdl.handle.net/10201/182069 | |
| dc.language | eng | |
| dc.publisher | Universidad de Murcia. Servicio de Publicaciones | |
| dc.relation | Sin financiación externa a la Universidad | |
| dc.relation.requires | https://doi.org/10.6018/analesbio | |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Aguas residuales | |
| dc.subject | Cloroformo | |
| dc.subject | Tasa de adsorción | |
| dc.subject | Siphoviridae | |
| dc.subject | Wastewater | |
| dc.subject | Chloroform | |
| dc.subject | Adsorption rate | |
| dc.subject | Siphoviridae | |
| dc.subject | Biocontrol | |
| dc.subject.ods | No relacionado con ningún objetivo de desarrollo sostenible | |
| dc.title | Isolation and characterization of FS01, a lytic bacteriophage infecting Salmonella typhimurium | |
| dc.title.alternative | Aislamiento y caracterización de FS01, un bacteriófago lítico que infecta a Salmonella typhimurium | |
| dc.type | info:eu-repo/semantics/article | |
| dspace.entity.type | Publication | es |
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