Divergence of cytokinesis and dimorphism control by myosin II regulatory light chain in fission yeasts.

Prieto Ruiz, Francisco; Gómez Gil, Elisa; Vicente Soler, Jero; Franco, Alejandro; Soto, Teresa; Madrid, Marisa; Cansado Vizoso, José

Publication:
Divergence of cytokinesis and dimorphism control by myosin II regulatory light chain in fission yeasts.

dc.contributor.author	Prieto Ruiz, Francisco
dc.contributor.author	Gómez Gil, Elisa
dc.contributor.author	Vicente Soler, Jero
dc.contributor.author	Franco, Alejandro
dc.contributor.author	Soto, Teresa
dc.contributor.author	Madrid, Marisa
dc.contributor.author	Cansado Vizoso, José
dc.contributor.department	Genética y Microbiología
dc.date.accessioned	2025-01-13T12:26:23Z
dc.date.available	2025-01-13T12:26:23Z
dc.date.issued	2023-09-15
dc.description	© 2023 Universidad de Murcia.. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted Manuscript version of a Published Work that appeared in final form in iScience. To access the final edited and published work see https://doi.org/10.1016/j.isci.2023.107611
dc.description.abstract	Non-muscle myosin II activation by regulatory light chain (Rlc1Sp) phosphorylation at Ser35 is crucial for cytokinesis during respiration in the fission yeast Schizosaccharomyces pombe. We show that in the early divergent and dimorphic fission yeast S. japonicus non-phosphorylated Rlc1Sj regulates the activity of Myo2Sj and Myp2Sj heavy chains during cytokinesis. Intriguingly, Rlc1Sj-Myo2Sj nodes delay yeast to hyphae onset but are essential for mycelial development. Structure-function analysis revealed that phosphorylation-induced folding of Rlc1Sp α1 helix into an open conformation allows precise regulation of Myo2Sp during cytokinesis. Consistently, inclusion of bulky tryptophan residues in the adjacent α5 helix triggered Rlc1Sp shift and supported cytokinesis in absence of Ser35 phosphorylation. Remarkably, unphosphorylated Rlc1Sj lacking the α1 helix was competent to regulate S. pombe cytokinesis during respiration. Hence, early diversification resulted in two efficient phosphorylation-independent and -dependent modes of Rlc1 regulation of myosin II activity in fission yeasts, the latter being conserved through evolution.	es
dc.format	application/pdf	es
dc.format.extent	48	es
dc.identifier.citation	iScience, 2023, Vol. 26, Issue 9 : 107611
dc.identifier.doi	https://doi.org/10.1016/j.isci.2023.107611
dc.identifier.issn	Electronic: 2589-0042
dc.identifier.uri	http://hdl.handle.net/10201/148336
dc.language	eng	es
dc.publisher	Cell Press
dc.relation	Ámbito del proyecto: Nacional Agencia financiadora: Agencia Estatal de Investigación Convocatoria: Proyectos de I+D+i, modalidad "generación de conocimiento". Nombre del proyecto: Mecanismos de control de la citocinesis modulados por señales ambientales Código o número del acuerdo de subvención: PID2020-112569GB-I00	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S2589004223016887?via%3Dihub
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología	es
dc.title	Divergence of cytokinesis and dimorphism control by myosin II regulatory light chain in fission yeasts.	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es