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Impact of seminal plasma antioxidants on DNA fragmentation and lipid peroxidation of frozen-thawed horse sperm

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dc.contributor.authorCatalán, Jaime
dc.contributor.authorYánez-Ortiz, Iván
dc.contributor.authorTorres-Garrido, Marc
dc.contributor.authorRibas-Maynou, Jordi
dc.contributor.authorLlavanera, Marc
dc.contributor.authorBarranco Cascales, Isabel
dc.contributor.authorYeste, Marc
dc.contributor.authorMiró, Jordi
dc.contributor.departmentMedicina y Cirugía Animal
dc.contributor.otherFacultad de Veterinaria
dc.date.accessioned2025-10-17T07:41:56Z
dc.date.available2025-10-17T07:41:56Z
dc.date.copyright© 2024 by the authors
dc.date.issued2024-03-06
dc.description.abstractCryopreservation is a stressful process for sperm, as it is associated with an increased production of reactive oxygen species (ROS). Elevated ROS levels, which create an imbalance with antioxidant capacity, may result in membrane lipid peroxidation (LPO), protein damage and DNA fragmentation. This study aimed to determine whether the membrane LPO and DNA fragmentation of frozen-thawed horse sperm relies upon antioxidant activity, including enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and paraoxonase type 1 (PON1)); non-enzymatic antioxidant capacity (Trolox-equivalent antioxidant capacity (TEAC), plasma ferric reducing antioxidant capacity (FRAP) and cupric reducing antioxidant capacity (CUPRAC)); and the oxidative stress index (OSI) of their seminal plasma (SP). Based on total motility and plasma membrane integrity (SYBR14+/PI-) after thawing, ejaculates were hierarchically (p < 0.001) clustered into two groups of good- (GFEs) and poor-(PFEs) freezability ejaculates. LPO and DNA fragmentation (global DNA breaks) were higher (p < 0.05) in the PFE group than in the GFE group, with LPO and DNA fragmentation (global DNA breaks) after thawing showing a positive relationship (p < 0.05) with SP OSI levels and ROS production. In addition, sperm motility and membrane integrity after thawing were negatively (p < 0.05) correlated with the activity levels of SP antioxidants (PON1 and TEAC). The present results indicate that LPO and DNA fragmentation in frozen-thawed horse sperm vary between ejaculates. These differences could result from variations in the activity of antioxidants (PON1 and TEAC) and the balance between the oxidant and antioxidant components present in the SP.
dc.formatapplication/pdf
dc.format.extent22
dc.identifier.citation Antioxidants 2024, 13, 322
dc.identifier.doihttps://doi.org/10.3390/antiox13030322
dc.identifier.eissn2076-3921
dc.identifier.urihttp://hdl.handle.net/10201/167310
dc.languageeng
dc.publisherMDPI
dc.relationJ.C. was funded by the Ministry of Universities (Spain) and by the European Union Next Generation EU Funds (Margarita Salas Scheme), and I.B. was funded by the Spanish Ministry of Science and Innovation (MCIN/AEI/10.13039/501100011033) and the European Union NextgenerationEU/PRTR (Ramón y Cajal Scheme: RYC2021-034546-I). The authors also acknowledge the support from the Ministry of Science and Innovation, Spain (Grant: PID2020-113320RB-I00); the Catalan Agency for the Management of University and Research Grants, Regional Government of Catalonia, Spain (Grant: 2017-SGR-1229 and 2021-SGR-00900); and the Catalan Institution for Research and Advanced Studies, Spain (ICREA).
dc.relation.publisherversionhttps://www.mdpi.com/2076-3921/13/3/322
dc.rightsAttribution 4.0 Internacional
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHorse
dc.subjectSemem
dc.subjectSeminal plasma
dc.subjectSperm
dc.subjectCryopreservation
dc.subjectAntooxidant
dc.subjectDNA fragmentation
dc.subjectLipid Peroxidation
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleImpact of seminal plasma antioxidants on DNA fragmentation and lipid peroxidation of frozen-thawed horse sperm
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublication3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
relation.isAuthorOfPublication.latestForDiscovery3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
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