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Saliva chromogranin A in growing pigs: a study of circadian patterns during daytime and stability under different storage conditions

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dc.contributor.authorEscribano Tortosa, Damián
dc.contributor.authorGutiérrez Montes, Ana María
dc.contributor.authorFuentes Rubio, María
dc.contributor.authorCerón Madrigal, José Joaquín
dc.contributor.departmentProducción Animal
dc.date.accessioned2025-01-15T13:10:55Z
dc.date.available2025-01-15T13:10:55Z
dc.date.issued2014-01-17
dc.description© 2014 Elsevier Ltd. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Veterinary Journal. To access the final edited and published work see https://doi.org/10.1016/j.tvjl.2014.01.005.es
dc.description.abstractSalivary chromogranin A (CgA) is considered to be a biomarker of activation of the sympatho-adrenome- dullary system, and has recently been proposed as a useful indicator of the acute stress response in pigs. The aim of the present study was to determinate whether salivary CgA concentrations in healthy growing pigs exhibits any circadian pattern during the daytime, and to evaluate its stability under different stor- age conditions. A total of 80 pigs (40 in spring and another 40 in autumn) of two different ages and gen- ders were used. To establish the circadian pattern, saliva samples were collected at 07.00, 11.00, 15.00 and 19.00 h on two consecutive days. Pooled samples were used for the stability study and were mea- sured on the day of sampling and periodically for up to 360 days later. Samples were stored at 4 °C, 20 °C or 80 °C and the effect of repeated freezing and thawing was also evaluated. No circadian pattern was detected for salivary CgA in either season and there were no significant effects of gender or age. However, mean salivary CgA concentrations were significantly higher (P < 0.0001) in the pigs sampled in autumn, compared to those sampled in the spring. Short term storage at 4 °C is recom- mended for up to 2 days, whereas frozen samples can be stored for 1 year at 20 °C or 80 °C, without substantial reduction in CgA values. In addition, samples can be frozen and thawed up to seven times without significant loss of the biomarker.es
dc.formatapplication/pdfes
dc.format.extent5es
dc.identifier.citationThe Veterinary Journal 199 (2014) 355–359
dc.identifier.doihttps://doi.org/10.1016/j.tvjl.2014.01.005
dc.identifier.issnPrint: 1090-0233
dc.identifier.issnElectronic: 1532-2971
dc.identifier.urihttp://hdl.handle.net/10201/148497
dc.languageenges
dc.publisherElsevieres
dc.relationÁmbito del proyecto: nacional. Agencia financiadora: MINISTERIO DE EDUCACION, CULTURA Y DEPORTE. Convocatoria: SUBPROGRAMA INVESTIGACIÓN FUNDAMENTAL NO ORIENTADA. -Nombre del proyecto: NUEVOS BIOMARCADORES DE ESTRÉS EN SALIVA DE GANADO PORCINO. Código o número del acuerdo de subvención: AGL 2009-08509.es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1090023314000148?via%3Dihub
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCircadian pattern
dc.subjectChromogranin A
dc.subjectSalivary proteins
dc.subjectStability
dc.subjectPigs
dc.subject.otherCDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticoses
dc.titleSaliva chromogranin A in growing pigs: a study of circadian patterns during daytime and stability under different storage conditionses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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