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Seva Alcaraz, Juan

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Seva Alcaraz, Juan
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Anatomía y Anatomía Patológica Comparadas
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Subject: Gene expression ×

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    Tomato Juice Supplementation Influences the Gene Expression Related to Steatosis in Rats
    (MDPI, 2018-09-02) Periago Castón, María Jesús; Elvira Torales, Laura Inés; Navarro González, Inmaculada; González Barrio, Rocío; Martín-Pozuelo, Gala; Domenech, Guillermo; García Alonso, Javier; Seva Alcaraz, Juan; Tecnología de Alimentos, Nutrición y Bromatología
    The objective of this work was to identify the effect of tomato juice on the expression of genes and levels of metabolites related to steatosis in rats. Male Sprague Dawley rats (8 weeks-old) were grouped (6 rats/group) in four experimental groups: NA (normal diet and water), NL (normal diet and tomato juice), HA (high-fat diet and water), and HL (high-fat diet and tomato juice). After an intervention period of 5 weeks, rats were sacrificed and biochemical parameters, biomarkers of oxidative stress, liver metabolites, and gene expression were determined. Although the H diet provoked dislipemia related to steatosis, no changes in isoprostanes or liver malondialdehyde (MDA) were observed. Changes in the gene expression of the HA group were produced by the high consumption of fat, whereas the consumption of tomato juice had different effects, depending on the diet. In the NL group, the genes involved in -oxidation were upregulated, and in groups NL and HL upregulation of CD36 and downregulation of APOB and LPL were observed. In addition, in the HL group the accumulation of lycopene upregulated the genes FXR and HNF4A, which have been suggested as preventive factors in relation to steatosis. Regarding the metabolomics study, intake of tomato juice stimulated the biosynthesis of glutathione and amino acids of the transulfurization pathway, increasing the levels of metabolites related to the antioxidant response.
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    Consumption of spinach and tomato modifies lipid metabolism, reducing Hepatic Steatosis in rats
    (MDPI, 2020-10-24) Elvira Torales, Laura Inés; Navarro González, Inmaculada; Rodrigo García, Joaquín; García Alonso, Javier; Periago Castón, María Jesús; Seva Alcaraz, Juan; Anatomía y Anatomía Patológica Comparadas
    Non-alcoholic fatty liver disease (NAFLD) is currently a serious and growing clinical problem in developed and developing countries and is considered one of the most frequent chronic liver diseases in the world. The aim of this study was to evaluate the functionality of dietary carotenoids provided by tomato and spinach in the dietary treatment of steatosis. Twenty-two Sprague-Dawley rats with induced steatosis were grouped into three groups and fed standard diet (CD group) and two experimental diets supplemented with 12.75% (LC12.75 group) and 25.5% (HC25.5 group) of a mixture of spinach and tomato powder. Rats fed carotenoid-rich feeds showed an improvement in the plasma biomarkers of steatosis, with lower levels of glucose, total cholesterol, VLDL, TG, proteins, ALT and AST. Likewise, a decrease in oxidative stress was observed, with a significant reduction of malondialdehyde (MDA) in plasma (up to 54%), liver (up to 51.42%) and urine (up to 78.89%) (p < 0.05) and an increase in plasma antioxidant capacity (ORAC) (up to 73.41%) (p < 0.05). Furthermore, carotenoid-rich diets led to an accumulation of carotenoids in the liver and were inversely correlated with the content of total cholesterol and hepatic triglycerides, increasing the concentrations of MUFA and PUFA (up to 32.6% and 48%, respectively) (p < 0.05). The accumulation of carotenoids in the liver caused the modulation of genes involved in lipid metabolism, and we particularly observed an overexpression of ACOX1, APOA1 and NRIH2 (LXR) and the synthesis of the proteins. This study suggests that dietary carotenoids from spinach and tomato aid in the dietary management of steatosis by reversing steatosis biomarkers.
  • Thumbnail Image
    Publication
    Open Access
    Tomato Juice Supplementation Influences the Gene Expression Related to Steatosis in Rats
    (MDPI, 2018-09-02) Elvira Torales, Laura Inés; Navarro González, Inmaculada; González Barrio, Rocío; Martín Pozuelo, Gala; Doménech, Guillermo; García Alonso, Javier; Periago Castón, María Jesús; Seva Alcaraz, Juan; Enfermería
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