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Moreno Ayuso, Juan Manuel

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Moreno Ayuso, Juan Manuel
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Universidad de Murcia. Departamento de Fisiología
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    In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds
    (Springer, 2019-06-15) Moreno Ayuso, Juan Manuel; Ramil‑Rego, Pablo; Gallego, Pedro Pablo; Barreal, M. Esther; García Pérez, Pascual; Tecnología de Alimentos, Nutrición y Bromatología; Facultad de Veterinaria
    Different Eryngium species have been used with ornamental, agricultural and medicinal purposes, as a consequence of their chemical constituents. In the southwest Europe the endemic Eryngium viviparum, presents a high risk of extinction and ex situ strategies are high recommended for efficient conservation and re-introduction program. The objective of this study was to satisfy a dual objective: (i) to develop an ex situ conservation strategy through micropropagation and (ii) taking advantage of the extraordinary potential of plant tissue culture, produce a considerable amount of plant material to carry out a preliminary phytochemical study, based on the accumulation of phenolic compounds and their associated antioxidant activity. First a factorial design was conducted in order to study the effect of two cytokinins (6- benzylaminopurine, BAP, and kinetin, KIN), at three levels (0, 1 and 2 mg L−1), on shoot multiplication. Later another factorial design was applied, by using three levels of MS medium salt strength (full, half and quarter- strength) and four sucrose levels (0, 1, 2, and 3%) for improving shoot elongation and rooting. In parallel, a preliminary quantification of total phenolic and flavonoid contents from E. viviparum aerial parts was determined. The simple micropropagation protocol designed allowed obtaining a high rates of shoot multiplication (5.1–5.8 new shoots), rooting (100%) with healthy long roots (3.1–3.5 cm) and plantlet acclimatization (96%). Moderate antioxidant activity was recorded in hydromethanolic extracts from E. viviparum aerial parts. High correlation between total phenolic content and BAP levels in the culture media was found. In conclusion, the micropropagation procedure described here for the endangered E. viviparum can be used as new and very efficient ex situ conservation strategy, and as potential source of antioxidants, conferring an added-value to this plant.
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    Capítulo 32. Regulación humoral de la función renal
    (McGraw-Hill, 2020) Llinás Más, María Teresa; Moreno Ayuso, Juan Manuel; Reverte Ribó, Virginia; Salazar Aparicio, Francisco Javier; Fisiología
    La función renal está regulada por un elevado número de hormonas estrechamente relacionadas entre sí, de modo que el funcionamiento del riñón es resultado del balance entre las acciones realizadas por estos factores humorales sobre la hemodinámica y la función excretora renal. Al considerar sus acciones, es preciso tener en cuenta que algunos de estos mecanismos no sólo tienen efectos directos, sino también efectos indirectos sobre la vasculatura renal y la reabsorción tubular. El estudio de los mecanismos que participan en el control de la función renal es de gran interés porque su alteración puede dar lugar a la aparición de diversas patologías, entre las que se puede mencionar la hipertensión y diversas nefropatías. En este capítulo se detalla la participación de los mecanismos que tienen mayor importancia en la regulación de la función renal, y se describen algunas de las interacciones existentes entre ellos. Dada su gran relevancia en el control de la función renal, el sistema renina-angiotensina (SRA) se describe con mayor detalle.
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