Blanco Carnero, José Eliseo

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Blanco Carnero, José Eliseo

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Blanco Carnero, José Eliseo

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Universidad de Murcia. Departamento de Cirugía, Pediatría, Obstetriciay Ginecología

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Open Access
Inherited epigenetic hallmarks of childhood obesity derived from prenatal exposure to obesogens
(MDPI, 2023-03-07) Núñez-Sánchez, María Á.; Jiménez-Méndez, Almudena; Suárez Cortés, María; Martínez-Sánchez, María A.; Sánchez-Solís de Querol, Manuel; Blanco Carnero, José Eliseo; Ruiz Alcaraz, Antonio José; Ramos-Molina, Bruno; Bioquímica y Biología Molecular B e Inmunología; Facultad de Biología
Childhood obesity has reached epidemic levels in developed countries and is becoming a major cause for concern in the developing world. The causes of childhood obesity are complex and multifactorial, involving the interaction between individual genetics and environmental and developmental factors. Among the environmental factors, there is a growing interest in understanding the possible relationship between the so-called environmental obesogens and the development of obesity in children. Exposure to these obesogens such as phthalates, bisphenol A, or parabens, has been identified as a promoter of obesity through different mechanisms such as the alteration of adipocyte development from mesenchymal progenitors, the interference with hormone receptors, and induced inflammation. However, less attention has been paid to the inheritance of epigenetic modifications due to maternal exposure to these compounds during pregnancy. Thus, the aim of this review is to summarize the current knowledge of epigenetic modifications due to maternal exposure to those obesogens during pregnancy as well as their potential implication on long-term obesity development in the offspring and transgenerational inheritance of epiphenotypes.
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In vivo kinetic study of materno-fetal fatty acid transfer in obese and normal weight pregnant women
(2019-10) Gázquez, Antonio; Van Harskamp, Dewin; Perazzolo, Simone; Oosterink, J. Efraim; Demmelmair, Hans; Schierbeek, Henk; Sengers, Bram G.; Lewis, Rohan M.; Van Goudoever, Johannes B.; Koletzko, Berthold; Larqué, Elvira; Blanco Carnero, José Eliseo; Prieto Sánchez, María Teresa; Cirugía, Pediatría y Obstetricia y Ginecología; Fisiología
Placental structure and function can be modified as a result of maternal obesity affecting materno-fetal fatty acids (FA) transport. We report for the first time, in humans and in vivo, the kinetics of placental FA transfer in normo-weight and in normolipemic obese pregnant women using stable isotopes. The administration of different tracer FA with similar behaviour to the mother at different time points allows the collection of kinetic information on materno-fetal transfer of FA despite only one sample of placenta and cord can be collected per subject. Computational modelling showed a good fit to the data when considering all maternal plasma lipid classes but not when based only on non-esterified FA. The novel approach using multiple tracer FA administration combined with computational modelling shows a consistent time course of placental tracer FA and predicted total FA accumulation.
Open Access
Influencia del sobrepeso y la obesidad en la transferencia placentaria de ácidos grasos en gestantes con diabetes gestacional.
(2013-07-01) Blanco Carnero, José Eliseo; Larqué Daza, Elvira; Parrilla Paricio, Juan José; Departamento de Fisiología
PALABRAS CLAVE: Diabetes gestacional, ácidos grasos poliinsaturados, transferencia placentaria, obesidad. El objetivo del estudio fue evaluar la influencia del sobrepeso y obesidad en las gestantes con diabetes gestacional, en su perfil de ácidos grasos en suero materno y en el aporte transplacentario al feto, comparándolo con gestantes normopeso con y sin diabetes gestacional. El sobrepeso y la obesidad en gestantes con DMG no modificó la concentración total de ácidos grasos o sus porcentajes, en suero materno ni en el tejido placentario. Sin embargo, en suero de vena umbilical la DMG con o sin obesidad disminuyó la concentración total de ácidos grasos fetales y los porcentajes de los ácidos grasos esenciales y condicionalmente esenciales. Como conclusión podemos afirmar que la diabetes gestacional con o sin obesidad disminuye los niveles de ácidos grasos poliinsaturados en sangre de cordón, lo cual puede ser debido a una alteración en el transporte placentario de estos nutrientes. KEY WORDS: Gestational diabetes mellitus, long-chain polyunsaturated fatty acids, placental transfer, obesity. The aim of the study was to evaluate the influence of overweight and obesity in pregnant women with gestational diabetes in their fatty acid profile in maternal serum and fetal transplacental intake, compared with normal weight pregnant women with and without gestational diabetes Overweight and obesity in pregnant women with gestational diabetes mellitus did not alter the total concentration of fatty acids or their percentage in maternal serum or placental tissue. However, in the umbilical vein serum with or without obesity gestational diabetes mellitus decreased total fatty acid concentration and the percentage of fetal essential fatty acids and conditionally essential. We can affirm that gestational diabetes mellitus, with or without overweight or obesity, reduces polyunsaturated fatty acids levels in cord blood, which might be due to impaired placental transport of these nutrients.
Open Access
Dynamics of the epigenome, microbiome, and metabolome in relation to early adiposity in the maternal–infant axis: protocol for a prospective, observational pilot study in the spanish NEMO cohort
(MDPI, 2025-09-23) Suárez Cortés, María; Juan-Pérez, Almudena; Molina Rodríguez, Alonso; Araújo de Castro, Julia; Castaño Molina, María de los Ángeles; Fernández Ruiz, Virginia Esperanza; Jiménez-Méndez, Almudena; Pérez-Munar, Paula Martínez; Rico-Chazarra, Sara; Ramos-Molina, Bruno; Sánchez-Solís de Querol, Manuel; Blanco Carnero, José Eliseo; Ruiz Alcaraz, Antonio José; Núñez-Sánchez, María Ángeles; Bioquímica y Biología Molecular B e Inmunología; Facultad de Biología
Background: Childhood obesity has reached epidemic levels in developed countries and is an emerging concern in developing regions. Children with excess weight are more likely to maintain this condition over time into adulthood and face a higher risk of developing metabolic disorders such as type 2 diabetes, hypertension, metabolic dysfunction-associated liver disease, and dyslipidemia. Early identification of obesity risk is, therefore, a key public health challenge. Methods: This is an observational, prospective, single-center cohort pilot study in 66 mother–infant dyads recruited at the Gynecology and Obstetrics Service of the Virgen de la Arrixaca University Hospital (Murcia, Spain). The primary objective is to identify early-life, non-invasive biomarkers associated with increased adiposity by integrating multi-omics approaches and analyzing maternal–infant interactions. Pregnant women will be enrolled during the third trimester and will undergo a baseline visit at 38 weeks of gestation for clinical and anthropometric assessment. Buccal swabs and fecal samples will be collected at baseline and in the peripartum period for epigenetic (DNA methylation), metagenomic, and metabolomic analyses. Infants will be evaluated at birth and followed at 6 months, 1 year, 2 years, and 3 years. Each visit will include detailed anthropometric measurements, along with collection of buccal swabs and fecal samples for multi-omics profiling. Conclusions: This multidisciplinary study aims to assess how maternal factors influence infant epigenetic and microbial patterns, and their relation to adiposity development. Early identification of such biomarkers may guide personalized prevention strategies and reduce the long-term burden of obesity-related comorbidities.
Open Access
Altered materno-fetal transfer of 13C-polyunsaturated fatty acids in obese pregnant women.
(2020-04) Gázquez, A.; Ruiz-Palacios, M.; van Harskamp, D.; Oosterink, J.E.; Schierbeek, H.; van Goudoever, J.B.; Demmelmair, H.; Koletzko, B.; Larqué, E.; Blanco Carnero, José Eliseo; Nieto Díaz, Anibal; Prieto Sánchez, María Teresa; Cirugía, Pediatría y Obstetricia y Ginecología
Maternal obesity at conception is considered a major predictor of offspring obesity. This could by driven at least in part by an altered placental fat transfer. However, the pathophysiological mechanisms involved are not fully understood. We investigated the in vivo materno-fetal transfer of fatty acids (FAs) in obese pregnant women using stable isotopes. Ten obese and ten normo-weight pregnant women (control) received orally a bolus of 13C-labeled FAs 12 h before elective caesarean section: oleic acid (13C-OA), linoleic acid (13C-LA) and docosahexaenoic acid (13C-DHA). Maternal blood samples were collected at -12 (basal), -8, -4, -2, 0 h relative to the time of cesarean section. At the time of birth, arterial and venous cord bloods as well as placental tissue were collected. FAs composition was determined by gas-liquid chromatography and isotopic enrichment by gas chromatography-combustion-isotope ratio mass spectrometry. Maternal plasma insulin and placental weight tended to higher values in obese pregnant women although they did not present serum hyperlipidemia. Higher concentrations of 13C-LA and 13C-DHA were found in non-esterified FAs fraction in maternal plasma of obese mothers. The ratio of placental uptake for 13C-LA and 13C-DHA was lower in obese women compared to normal weight pointing toward a limited capacity of FA placental transfer, especially of essential FAs. Maternal insulin was associated to this lower placenta/maternal plasma ratio for both 13C-LA (R = -0.563, P = 0.012) and 13C-DHA (R = -0.478, P = 0.033). In addition, the ratio cord/maternal plasma of 13C-LA was significantly lower in obese women compared to controls. In conclusion, obese mothers without hyperlipidemia showed a reduced materno-fetal transfer of polyunsaturated FAs which could affect fetal development. This affect dietary recommendation for obese pregnant women.
Open Access
Child head circumference and placental MFSD2a expression are associated to the level of MFSD2a in maternal blood during pregnancy
(Frontiers Media, 2020-02-05) Sánchez-Campillo, María; Ruiz-Palacios, María; Ruiz Alcaraz, Antonio José; Blanco Carnero, José Eliseo; Prieto Sánchez, María Teresa; Zornoza, Matilde; Ruiz-Pastor, María José; Demmelmair, Hans; Sánchez-Solís de Querol, Manuel; Koletzko, Berthold; Larqué Daza, Elvira; Bioquímica y Biología Molecular B e Inmunología; Facultad de Biología
Gestational diabetes mellitus (GDM) is a world-wide health challenge, which prevalence is expected to increase in parallel to the epidemic of obesity. Children born from GDM mothers have lower levels of docosahexaenoic acid (DHA) in cord blood, which might influence their neurodevelopment. Recently, the membrane transporter Major Family Super Domain 2a (MFSD2a) was associated with the selective transportation of DHA as lysophospholipids. The expression of the DHA membrane transporter MFSD2a is lower in GDM placentas, which could affect materno-fetal DHA transport. Humans with homozygous inactivating mutations in the MFSD2a gene present severe microcephalyand intellectual impairments. Herein, we intended to identify early blood biomarkers that maybeofuse during pregnancy to monitor the offspring development and the adequate nutritional interventions, such as nutritional supplementation, that may be selected to improve it. We evaluated MFSD2a expression in maternal blood at the third trimester of pregnancy, and its potential relationship with the expression of placental MFSD2a at delivery and child outcomes. Three groups of pregnant women were recruited: 25 controls, 23 GDM with dietary treatment, and 20 GDM with insulin treatment. Maternal and neonatal anthropometric and biochemical parameters were evaluated. MFSD2a was analyzed in placenta, blood and serum. MFSD2a protein expression in maternal blood was significantly lower in GDM groups and correlated with placental MFSD2a and Z-score neonatal head circumference during the first 6 months of life. The cord/maternal serum ratio of DHA, a solid indicator of materno-fetal DHA transport, was reduced in GDMgroups and correlated with MFSD2a in maternal blood at the third trimester and in placenta at delivery. This indicates that altered MFSD2a levels in maternal blood during pregnancy might influence placental nutrient transport and fetal neurodevelopment. Furthermore, MFSD2a levels in maternal blood on the third trimester were inversely correlated to DHA in maternal serum lyso-PL. Thus, the level of MFSD2a in maternal blood could be used as a potential biomarker for the early detection of disturbances of MFSD2a expression during pregnancy and the subsequent consequences for the neurodevelopment of the child, as well as it may help to choose the optimal treatment approach for the affected subjects.
Open Access
Child Head Circumference and Placental MFSD2a Expression Are Associated to the Level of MFSD2a in Maternal Blood During Pregnancy
Sanchez-campillo Muñoz, M.; Ruiz Palacios, M.; Zornoza Moreno, M.; Ruiz Pastor, M. J.; Demmelmair, H.; Koletzko, B.; Larqué Daza, Elvira; Ruiz Alcaraz, Antonio José; Blanco Carnero, José Eliseo; Prieto Sánchez, María Teresa; Sánchez-Solís de Querol, Manuel; Cirugía, Pediatría y Obstetricia y Ginecología